In vitro cloning and homestead cultivation of primitive Musa cultivars.

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dc.contributor.authorMukunthakumar, Sen_US
dc.contributor.authorSeeni, Sen_US
dc.date.accessioned2009-05-28T13:45:06Z
dc.date.available2009-05-28T13:45:06Z
dc.date.issued2005-01-05en_US
dc.description.abstractTwo primitive diploid Musa cultivars, Matti and Chemmatti from the extreme southern part of the Western Ghats were multiplied by in vitro culture of sucker-derived shoot apices. Decontaminated corm explants (1 cm x 1 cm) having shoot apex (approximately 0.3 cm) cultured for 1 month in Murashige and Skoog basal agar medium was cut vertically into eight segments and each segment having a part of shoot meristem was cultured in presence of 6-benzylaminopurine (BAP) and combinations of BAP and indole-3-acetic acid (IAA) or indole-3-butyricacid (IBA) to produce multiple shoots. After 12 weeks of culture, maximum number of shoots (32) in both the cultivars were produced in approximate 60% of the explants in presence of BAP and IAA each at 1.5 mg/l(-1) (Matti) and 40% of the explants in 2.5 mg/l(-1) of BAP and 1.5 mg/l(-1) of IAA (Chemmatti). Buds were formed from the base of the subcultured shoots and somewhat more number (34) of shoots were obtained in Matti than in Chemmatti (31) after 8 weeks. Difference in the concentration of cytokinin required for shoot initiation and multiplication, persistence of exudation through the subculture and red colouration of the early formed sheathing leaf bases in the shoots in Chemmatti indicated possible genotypic differences between the two cultivars. Multiple shoot proliferation achieved through five subcultures of the isolated shoots without any decline. Transfer of shoots (4-5 cm) into MS basal medium favoured rooting in 4 weeks and rooted plants (9 cm) were hardened and established (80-95%). Mericlones of Matti cultivated in homesteads produced bunches of uniform characters in 13 months.en_US
dc.description.affiliationTropical Botanic Garden and Research Institute, Pacha Palode, Thiruvananthapuram 695 562, India.en_US
dc.identifier.citationMukunthakumar S, Seeni S. In vitro cloning and homestead cultivation of primitive Musa cultivars. Indian Journal of Experimental Biology. 2005 Jan; 43(1): 90-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/59818
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAdenine --analogs & derivativesen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshGenotypeen_US
dc.subject.meshIndoleacetic Acids --pharmacologyen_US
dc.subject.meshIndoles --pharmacologyen_US
dc.subject.meshKinetinen_US
dc.subject.meshMeristem --geneticsen_US
dc.subject.meshMusa --geneticsen_US
dc.subject.meshPlant Growth Regulators --pharmacologyen_US
dc.titleIn vitro cloning and homestead cultivation of primitive Musa cultivars.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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