Molecular Analysis of Genetic Variability and Relationship among Pearl Millet (Pennisetum glaucum) Genotypes by Using SSR Markers

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dc.contributor.authorKasar, V. B.en_US
dc.contributor.authorSurbhaiyya, S. D.en_US
dc.contributor.authorNikam, S. D.en_US
dc.contributor.authorSutar, S. R.en_US
dc.contributor.authorKasar, A. S.en_US
dc.contributor.authorPorje, D. S.en_US
dc.date.accessioned2024-09-24T07:25:13Z
dc.date.available2024-09-24T07:25:13Z
dc.date.issued2023-08
dc.description.abstractPearl millet has a place with sort Pennisetum, and family Poaceae. Pearl millet is the most broadly developed grain crop in Asia and Africa representing close to half of the worldwide millet creation. In India, pearl millet is the fourth most broadly developed food crop after rice, wheat, and maize. We used molecular techniques to investigate the genetic diversity and relatedness of six genotypes viz:ICMV155, Dhanshakti, PC-612, Sampada, HHB-67, and HHB-197.the six genotypes was collected from National agriculture research project Aurangabad. The present study was conducted at the Department of Plant Biotechnology at K. K. Wagh College of Agricultural Biotechnology, Nashik.DNA was isolated by fixing a sample in alcohol without using liquid nitrogen; six genotypes were analyzed through SSR primers to determine the extent of molecular characterization. PCR amplification using 10 SSR primers generated a total of 111 no. of bands were scored corresponding an average of 11 bands per primer with 77 bands showing polymorphism (67%) and 34 bands showing monomorphism (30%). The PIC value ranged from 0.35 to 0.68 with an average of 0.4. Jaccard’s coefficient based on SSR analysis 0.38 to 0.90. The dendrogram wasconstructed using the UPGMA method. It has two main clusters Cluster-1 consisting of C1- HHB 67, V6-ICMV-155, C2- HHB 197 and C4- Sampada. Cluster-2 comprised C3-Dhanshakti and V5-PC 612 as an out group. C2- HHB 197 and C4- Sampada genotypes have the highest similarity coefficient 0.76. Among all the genotypes, C1- HHB 67 and V5- PC 612 was found most diverse as it separated from all other genotypes at a very low similarity coefficient of 0.6. The identified markers can prove useful for identification of diverse germplasm and future DNA fingerprinting studies.en_US
dc.identifier.affiliationsDepartment of Plant Biotechnology, K.K. Wagh College of Agricultural Biotechnology, Nashik- 422003, Indiaen_US
dc.identifier.affiliationsDepartment of Plant Biotechnology, K.K. Wagh College of Agricultural Biotechnology, Nashik- 422003, Indiaen_US
dc.identifier.affiliationsDepartment of Plant Biotechnology, K.K. Wagh College of Agricultural Biotechnology, Nashik- 422003, Indiaen_US
dc.identifier.affiliationsDepartment of Plant Biotechnology, K.K. Wagh College of Agricultural Biotechnology, Nashik- 422003, Indiaen_US
dc.identifier.affiliationsDepartment of Plant Biotechnology, K.K. Wagh College of Agricultural Biotechnology, Nashik- 422003, Indiaen_US
dc.identifier.affiliationsDepartment of Plant Biotechnology, K.K. Wagh College of Agricultural Biotechnology, Nashik- 422003, Indiaen_US
dc.identifier.citationKasar V. B., Surbhaiyya S. D., Nikam S. D., Sutar S. R., Kasar A. S., Porje D. S. . Molecular Analysis of Genetic Variability and Relationship among Pearl Millet (Pennisetum glaucum) Genotypes by Using SSR Markers. International Journal of Environment and Climate Change. 2023 Aug; 13(8): 1068-1076en_US
dc.identifier.issn2581-8627
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/229130
dc.languageenen_US
dc.publisherMs. M. B. Mondalen_US
dc.relation.issuenumber8en_US
dc.relation.volume13en_US
dc.source.urihttps://doi.org/10.9734/ijecc/2023/v13i82045en_US
dc.subjectPearl milleten_US
dc.subjectDNAen_US
dc.subjectRNaseen_US
dc.subjectgel electrophoresisen_US
dc.subjectSSR markersen_US
dc.subjectDNA profilingen_US
dc.titleMolecular Analysis of Genetic Variability and Relationship among Pearl Millet (Pennisetum glaucum) Genotypes by Using SSR Markersen_US
dc.typeJournal Articleen_US
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