Functional aspect of a phosphopeptide derived from calf thymus nuclei and DNA.

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dc.contributor.authorWelsh, R Sen_US
dc.contributor.authorVyska, Ken_US
dc.contributor.authorDohmen, Wen_US
dc.contributor.authorMielke, Ten_US
dc.contributor.authorChakravorty, Ren_US
dc.contributor.authorMukherjee, A Sen_US
dc.date.accessioned2009-05-28T13:01:19Z
dc.date.available2009-05-28T13:01:19Z
dc.date.issued1990-04-01en_US
dc.description.abstractPhosphopeptides (PPs) isolated from highly purified calf thymus DNA (N-DNA) and extracted from calf thymus nuclei were fractionated, and the effect of one PP fraction on DNA replication has been examined. In the absence of inhibitors, the increasing PP concentration caused a linear decrease of 3H-thymidine uptake in L5178Y cells. If PP fraction was mildly hydrolysed with 1NHCl, the decrease in uptake was much steeper. The studies in which the inhibitors were used revealed that by the addition of the unhydrolysed PP fraction the inhibition of 3H-thymidine uptake by alpha-amanitin could be completely overcome, and that the inhibition by puromycin was reduced to 65-77% of the control. With puromycin, there was a gradual decrease of 3H-thymidine uptake with PP concentration above 3 mg/ml. The PPs gave an increase in incorporation of 3H-thymidine even after removal of alpha-amanitin and puromycin; thus, it is suggested that there is no direct interaction of either inhibitor with PP in the cell. Data on the utilization of 3H-cytidine for the synthesise of new DNA suggest that PP fraction might cause an acceleration of DNA replication.en_US
dc.description.affiliationInstitute of Medicine, Nuclear Research Center, Julich, FRG.en_US
dc.identifier.citationWelsh RS, Vyska K, Dohmen W, Mielke T, Chakravorty R, Mukherjee AS. Functional aspect of a phosphopeptide derived from calf thymus nuclei and DNA. Indian Journal of Experimental Biology. 1990 Apr; 28(4): 301-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/58858
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCattleen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCell Nucleus --metabolismen_US
dc.subject.meshChromatography, Ion Exchangeen_US
dc.subject.meshDNA --analysisen_US
dc.subject.meshDNA Replicationen_US
dc.subject.meshPhosphopeptides --isolation & purificationen_US
dc.subject.meshThymidine --metabolismen_US
dc.subject.meshThymus Glanden_US
dc.titleFunctional aspect of a phosphopeptide derived from calf thymus nuclei and DNA.en_US
dc.typeJournal Articleen_US
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