DNA extraction from crayfish exoskeleton.

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dc.contributor.authorLi, Yanhe
dc.contributor.authorWang, Weimin
dc.contributor.authorLiu, Xiaolian
dc.contributor.authorLuo, Wei
dc.contributor.authorZhang, Jie
dc.contributor.authorGul, Yasmeen
dc.date.accessioned2013-02-22T05:59:26Z
dc.date.available2013-02-22T05:59:26Z
dc.date.issued2011-12
dc.description.abstractCrayfish exoskeleton (CE) samples are generally less invasive and easy to be collected. However, it is difficult to extract DNA from them. This study was intended to investigate CE as a DNA source and design an easy and efficient DNA extraction protocol for polymerase chain reactions. Specific primer pair (PPO-F, PPO-R) was used to amplify extracted DNA from CE, and compared to crayfish tail muscle DNA sample. Moreover, seven microsatellites markers were used to amplify the CE DNA samples set. Since the extracted DNA from CE is suitable for gene amplification, the results present usefulness of CE as an easy and convenient DNA source for PCR-based population genetic research.en_US
dc.identifier.citationLi Yanhe, Wang Weimin, Liu Xiaolian, Luo Wei, Zhang Jie, Gul Yasmeen. DNA extraction from crayfish exoskeleton. Indian Journal of Experimental Biology. 2011 Dec; 49(12): 953-957.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/145214
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/13094en_US
dc.subjectCrayfish exoskeletonen_US
dc.subjectDNA amplificationen_US
dc.subjectDNA extractionen_US
dc.titleDNA extraction from crayfish exoskeleton.en_US
dc.typeArticleen_US
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