In vitro brain tyrosine hydroxylase activation in catfish Heteropneustes fossilis (Bloch): seasonal changes in involvement of cAMP-dependent protein kinase A and Ca2+ -dependent protein kinase C.

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dc.contributor.authorChaube, Ren_US
dc.contributor.authorJoyt, K Pen_US
dc.date.accessioned2009-05-28T15:11:02Z
dc.date.available2009-05-28T15:11:02Z
dc.date.issued2008-11-19en_US
dc.description.abstractIn the present in vitro study, the involvement of cAMP dependent-protein kinase A (PKA) and calcium-dependent protein kinase C (PKC) in the regulation of forebrain (telencephalon and hypothalamus) tyrosine hydroxylase (TH) activity was demonstrated during the reproductive seasons of the female catfish H. fossilis. In the concentration studies conducted in prespawning phase, cAMP (0.05 nM, 0.5 nM, 1 mM and 2.0 mM) or the phosphodiesterase inhibitor isobutylmethylxanthine (IBMX-0.5-2.0 mM) stimulated enzyme activity. Likewise, the incubation of the enzyme preparations with the cAMP dependent-protein kinase A inhibitor H-89 (1 and 10 microM) and PKC inhibitor calphostin C (cal C; 1 and 10 microM) inhibited enzyme activity in a concentration-dependent manner. In seasonal studies, the incubation of the enzyme preparations with cAMP (1 mM), IBMX (1 mM), H-89 (10 microM) and cal-C (10 microM) produced season-dependent effects on enzyme activity. The stimulatory effect of cAMP and IBMX and the inhibitory effect of H-89 and cal C were greater in the resting and spawning phases. The results suggest the involvement of both signal transduction pathways in TH activation vis-à-vis catecholaminergic activity with a more dominant role by the cAMP-PKA pathway.en_US
dc.description.affiliationDepartment of Zoology, Banaras Hindu University, Varanasi 221 005, India.en_US
dc.identifier.citationChaube R, Joyt KP. In vitro brain tyrosine hydroxylase activation in catfish Heteropneustes fossilis (Bloch): seasonal changes in involvement of cAMP-dependent protein kinase A and Ca2+ -dependent protein kinase C. Indian Journal of Experimental Biology. 2008 Nov; 46(11): 764-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/61661
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/4646/1/IJEB%2046%2811%29%20764-769.pdfen_US
dc.subject.mesh1-Methyl-3-isobutylxanthine --pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain --enzymologyen_US
dc.subject.meshCalcium --metabolismen_US
dc.subject.meshCatfishesen_US
dc.subject.meshCyclic AMP --metabolismen_US
dc.subject.meshCyclic AMP-Dependent Protein Kinases --metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshFossilsen_US
dc.subject.meshIsoquinolines --pharmacologyen_US
dc.subject.meshNaphthalenes --pharmacologyen_US
dc.subject.meshProtein Kinase C --metabolismen_US
dc.subject.meshSeasonsen_US
dc.subject.meshSignal Transductionen_US
dc.subject.meshSulfonamides --pharmacologyen_US
dc.subject.meshTyrosine 3-Monooxygenase --chemistryen_US
dc.titleIn vitro brain tyrosine hydroxylase activation in catfish Heteropneustes fossilis (Bloch): seasonal changes in involvement of cAMP-dependent protein kinase A and Ca2+ -dependent protein kinase C.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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