Cytotoxicity of Aqueous and Ethanolic Extracts of Ficus deltoidea on Human Ovarian Carcinoma Cell Line.

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dc.contributor.authorAkhir, Nor Azurah Mat
dc.contributor.authorChua, Lee Suan
dc.contributor.authorMajid, Fadzilah Adibah Abdul
dc.contributor.authorSarmidi, Mohamad Roji
dc.date.accessioned2015-08-31T05:13:23Z
dc.date.available2015-08-31T05:13:23Z
dc.date.issued2011-10
dc.description.abstractAims: This study was to investigate the cytotoxicity of both plant extracts from Ficus deltoidea (locally known as Mas Cotek), aqueous and ethanolic extracts on human ovarian carcinoma cells using standard colometric MTT assay. Study design: Cell based assay Place and Duration of Study: Institute of Bioproduct Development and Department of Bioprocess Engineering, Universiti Teknologi Malaysia, Johor Bahru, Malayisa between January 2007 and December 2009. Methodology: The biochemical responses of cells after plant sample treatment were observed and have been reported through several assays such as trypan blue exclusion assay for cell viability, analysis of glucose uptake and lactate release, cell survival evaluation and genomic assay through DNA fragmentation. Results: Both aqueous and ethanolic extracts of the plant sample gave IC50 value of 224.39 + 6.24 μg/ml and 143.03 ± 20.21 μg/ml, respectively. The detachment capability of the plant aqueous extract was observed in the cell viability assays. DNA fragmentation was not observed in the aqueous extract, but in ethanolic extract (1000 μg/ml). The DNA was fragmented around 200 Kbp. Morphological observation was carried out and apoptosis body was observed at 1000 μg/ml of both extract. Conclusion: A2780 cancer cells behaved differently on cell growth profile upon treating with different concentrations of the aquoues and ethanolic extracts of F. deltoidea. Even though both extracts could cause apoptosis at 1000 μg/ml, the aqueous extract prompted to promote cell detachment, and the ethanolic tried to inhibit cell proliferation through DNA fragmentation.en_US
dc.identifier.citationAkhir Nor Azurah Mat, Chua Lee Suan, Majid Fadzilah Adibah Abdul, Sarmidi Mohamad Roji. Cytotoxicity of Aqueous and Ethanolic Extracts of Ficus deltoidea on Human Ovarian Carcinoma Cell Line. British Journal of Medicine and Medical Research. 2011 Oct; 1(4): 397-409.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/162748
dc.language.isoenen_US
dc.source.urihttps://sciencedomain.org/abstract/229en_US
dc.subjectFicus deltoideaen_US
dc.subjecthuman ovarian carcinoma cellsen_US
dc.subjectcytotoxicityen_US
dc.subjectDNA fragmentationen_US
dc.titleCytotoxicity of Aqueous and Ethanolic Extracts of Ficus deltoidea on Human Ovarian Carcinoma Cell Line.en_US
dc.typeArticleen_US
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