Staphylococcus aureus colonization of anterior nares of school going children.

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dc.contributor.authorRamana, K V
dc.contributor.authorMohanty, S K
dc.contributor.authorWilson, C G
dc.date.accessioned2012-10-22T07:28:49Z
dc.date.available2012-10-22T07:28:49Z
dc.date.issued2009-08
dc.description.abstractObjective. To determine the colonization rates of S. aureus in anterior nares of school going children, evaluate the antimicrobial resistance of such isolates against various antibiotics. Methods. Nasal swabs taken for S.aureus in 392 healthy school going children aged between 5 and 15 yr belonging to three schools surrounding Kamineni Institute of Medical Sciences, Narketpally, Andhra Pradesh, India. Swabs were inoculated in to Mannitol Salt Agar (MSA) and incubated at 37o C for 24hr, Staphylococcus aureus isolates were identified by standard microbiological methods such as Gram’s stain, catalase and coagulase. MICs were determined by Agar dilution technique against Vancomycin, Ciprofloxacin, Sparfloxacin, Sparfloxacin ß Cyclodextrin. Antimicrobial resistance patterns of all the isolates against Oxacillin (1 μg) Penicillin (10units), Ampicillin (10μg), Co-trimoxazole (23.75 μg), Erythromycin (15 μg), Tetracycline (30 μg) and Gentamicin (10 μg) were tested using Kirby-Bauer disk diffusion method in accordance with CLSI standards. Results. Of the 392 samples screened 63(16%) showed the growth of Staphylococcus aureus. Twelve (19%) isolates were found to be MRSA. Antimicrobial susceptibility testing using Agar dilution method against Vancomycin, ciprofloxacin , Sparfloxacin, and Sparfloxacin β Cyclodextrin revealed MICs in the range of 0.52 μg/ml, 0.51 μg/ml, 0.5 μg/ml and <0.0312- 0.250 μg/ml respectively. Disk diffusion method showed that all the isolates were resistant to Penicillin and Ampicillin. A resistance of 14.3%, 25% and 22.2% was observed against Co-trimoxazole, Erythromycin and Tetracycline respectively. Gentamiicin was the only antibiotic against which most of the isolates were sensitive. Conclusion. Our results suggest that healthy school going children under 16 yr of age are potential carriers of S. aureus and in particular MRSA and multi-drug resistant strains.en_US
dc.identifier.citationRamana K V, Mohanty S K, Wilson C G. Staphylococcus aureus colonization of anterior nares of school going children. Indian Journal of Pediatrics. 2009 Aug; 76(8): 813-816.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/142346
dc.language.isoenen_US
dc.source.urihttps://medind.nic.in/icb/t09/i8/icbt09i8p813.pdfen_US
dc.subjectS. aureusen_US
dc.subjectMethicillin resistant staphylococcus aureus (MRSA)en_US
dc.subjectColonizationen_US
dc.subject.meshAdolescent
dc.subject.meshAnti-Bacterial Agents --pharmacology
dc.subject.meshCarrier State
dc.subject.meshChild
dc.subject.meshChild, Preschool
dc.subject.meshDrug Resistance, Bacterial
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshIndia
dc.subject.meshMale
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshNose --microbiology
dc.subject.meshStaphylococcus aureus --drug effects
dc.subject.meshStaphylococcus aureus --isolation & purification
dc.titleStaphylococcus aureus colonization of anterior nares of school going children.en_US
dc.typeArticleen_US
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