Staphylococcus aureus colonization of anterior nares of school going children.
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Date
2009-08
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Abstract
Objective. To determine the colonization rates of S. aureus in anterior nares of school going children, evaluate the
antimicrobial resistance of such isolates against various antibiotics.
Methods. Nasal swabs taken for S.aureus in 392 healthy school going children aged between 5 and 15 yr belonging to
three schools surrounding Kamineni Institute of Medical Sciences, Narketpally, Andhra Pradesh, India. Swabs were
inoculated in to Mannitol Salt Agar (MSA) and incubated at 37o C for 24hr, Staphylococcus aureus isolates were identified
by standard microbiological methods such as Gram’s stain, catalase and coagulase. MICs were determined by Agar
dilution technique against Vancomycin, Ciprofloxacin, Sparfloxacin, Sparfloxacin ß Cyclodextrin. Antimicrobial resistance
patterns of all the isolates against Oxacillin (1 μg) Penicillin (10units), Ampicillin (10μg), Co-trimoxazole (23.75 μg),
Erythromycin (15 μg), Tetracycline (30 μg) and Gentamicin (10 μg) were tested using Kirby-Bauer disk diffusion method in
accordance with CLSI standards.
Results. Of the 392 samples screened 63(16%) showed the growth of Staphylococcus aureus. Twelve (19%) isolates were
found to be MRSA. Antimicrobial susceptibility testing using Agar dilution method against Vancomycin, ciprofloxacin ,
Sparfloxacin, and Sparfloxacin β Cyclodextrin revealed MICs in the range of 0.52 μg/ml, 0.51 μg/ml, 0.5 μg/ml and <0.0312-
0.250 μg/ml respectively. Disk diffusion method showed that all the isolates were resistant to Penicillin and Ampicillin. A
resistance of 14.3%, 25% and 22.2% was observed against Co-trimoxazole, Erythromycin and Tetracycline respectively.
Gentamiicin was the only antibiotic against which most of the isolates were sensitive.
Conclusion. Our results suggest that healthy school going children under 16 yr of age are potential carriers of S. aureus and
in particular MRSA and multi-drug resistant strains.
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Keywords
S. aureus, Methicillin resistant staphylococcus aureus (MRSA), Colonization
Citation
Ramana K V, Mohanty S K, Wilson C G. Staphylococcus aureus colonization of anterior nares of school going children. Indian Journal of Pediatrics. 2009 Aug; 76(8): 813-816.