Antibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica WALL.

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dc.contributor.authorKavitha, Den_US
dc.contributor.authorShilpa, P Nen_US
dc.contributor.authorDevaraj, S Niranjalien_US
dc.date.accessioned2009-05-28T10:57:08Z
dc.date.available2009-05-28T10:57:08Z
dc.date.issued2004-06-21en_US
dc.description.abstractThe alkaloids from the ethanolic extract of H. antidysenterica seeds were evaluated for their antibacterial activity against clinical isolates of enteropathogenic Escherichia coli (EPEC) in vitro, and their antidiarrhoeal activity on castor oil-induced diarrhoea in rats, in vivo. The plasmid DNA, whole cell lysate and outer membrane protein profile of a clinical isolate of EPEC was determined in presence of alkaloids of H. antidysenterica. The disc diffusion and agar well diffusion methods were used to evaluate the antibacterial efficacy. The alkaloids showed strong antibacterial activity against EPEC strains. In castor oil-induced diarrhoea, alkaloids reduced the diarrhoea with decrease in the number of wet faeces in pretreated rats at a dose of 200-800 mg/kg. The loss of plasmid DNA and suppression of high molecular weight proteins were observed on alkaloids treatment. Taking into account the multiple antibiotic resistance of EPEC, the results suggest usefulness of alkaloids of H. antidysenterica seeds as antibacterial and antidiarrhoeal agents.en_US
dc.description.affiliationDepartment of Biochemistry, University of Madras, Guindy Campus, Chennai 600 025, India.en_US
dc.identifier.citationKavitha D, Shilpa PN, Devaraj SN. Antibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica WALL. Indian Journal of Experimental Biology. 2004 Jun; 42(6): 589-94en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56029
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAgar --chemistryen_US
dc.subject.meshAlkaloids --metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnti-Bacterial Agents --pharmacologyen_US
dc.subject.meshAntidiarrheals --pharmacologyen_US
dc.subject.meshCastor Oil --metabolismen_US
dc.subject.meshDiffusionen_US
dc.subject.meshEscherichia coli --metabolismen_US
dc.subject.meshFeces --microbiologyen_US
dc.subject.meshHolarrhena --metabolismen_US
dc.subject.meshPlant Extracts --pharmacologyen_US
dc.subject.meshPlasmids --metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshTemperatureen_US
dc.titleAntibacterial and antidiarrhoeal effects of alkaloids of Holarrhena antidysenterica WALL.en_US
dc.typeJournal Articleen_US
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