Application of polymerase chain reaction (PCR) for diagnosis of equine herpes virus-1 (EHV-1).
No Thumbnail Available
Date
1996-11-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Fifty aborted foetus samples were diagnosed for the presence of equine herpes virus-1 (EHV-1) by polymerase chain reaction (PCR) technique. Specific primer pair for amplification of a particular segment of EHV-1 DNA in gc region having 3 Hae III restriction endonuclease sites was used. A 409 base pair segment obtained as PCR amplification product in 9 samples was digested with Hae III to confirm the presence of EHV-1 as the infectious agent in aborted tissues. It was observed that PCR technique was more sensitive, specific and rapid than the conventional virological diagnostic methods.
Description
Keywords
Citation
Gupta AK, Singh BK, Yadav MP. Application of polymerase chain reaction (PCR) for diagnosis of equine herpes virus-1 (EHV-1). Indian Journal of Experimental Biology. 1996 Nov; 34(11): 1077-80