Decolorization of Synthetic Dyes by Ficus carica Latex Peroxidase Isoenzymes

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dc.contributor.authorElsayed, Alshaimaa M.en_US
dc.contributor.authorHegazy, Usama M.en_US
dc.contributor.authorHegazy, Marwa G. A.en_US
dc.contributor.authorAbdel-Ghany, Somia S.en_US
dc.contributor.authorSalama, Walaa H.en_US
dc.contributor.authorSalem, Ahmed M. H.en_US
dc.contributor.authorFahmy, Afaf S.en_US
dc.date.accessioned2019-12-06T09:41:13Z
dc.date.available2019-12-06T09:41:13Z
dc.date.issued2018-07
dc.description.abstractAims: The current study aims to elucidate the potential of Ficus carica latex peroxidase isoenzymes for decolorizing different synthetic dyes in comparison to the commercial horseradish peroxidase. Study Design: The decolorization of 20 dyes was investigated using the purified F. carica latex peroxidase isoenzymes (purified FP1 and partially purified FP2, and FP3), and horseradish peroxidase (HRP) as a control. Place and Duration of Study: Molecular Biology Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Egypt, between January 2017 and March 2018. Methodology: The purified and partially fractions of peroxidase isolated from latex of F. carica were used for the present study. Stock solutions of the dyes were prepared in 0.05 M sodium acetate buffer (pH 5.5) and diluted to the requested concentrations ranged from 12 to 330 µM in order to get maximum absorbance does not exceed 1.5 as initial reading. The efficiency of decolorization was expressed in terms of percentage. All experiments were performed in triplicate. Results: F. carica latex peroxidase isoenzymes and commercial horseradish peroxidase were able to decolorize some of tested dyes and the extent of decolorization achieved with different dyes classes were varied according to different chemical structure of each dye. The decolorization efficiency after 3 h of incubation at 40°C using 6.4 U/ml of peroxidase activity of FP1, FP2, FP3 and HRP, was found to be extremely efficient in decolorizing some dyes and relatively low in other dyes. Conclusion: The efficiency of F. carica latex peroxidase isoenzymes toward different synthetic dyes meet the prerequisites needed for environmental and industrial applications.en_US
dc.identifier.affiliationsDepartment of Molecular Biology, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, 12622 Giza, Egypten_US
dc.identifier.affiliationsDepartment of Molecular Biology, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, 12622 Giza, Egypten_US
dc.identifier.affiliationsDepartment of Biochemistry, Faculty of Science, Ain Shams University, P.O.B 11381, Abbassia, Cairo, Egypten_US
dc.identifier.affiliationsDepartment of Molecular Biology, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, 12622 Giza, Egypten_US
dc.identifier.affiliationsDepartment of Molecular Biology, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, 12622 Giza, Egypten_US
dc.identifier.affiliationsDepartment of Biochemistry, Faculty of Science, Ain Shams University, P.O.B 11381, Abbassia, Cairo, Egypten_US
dc.identifier.affiliationsDepartment of Molecular Biology, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, 12622 Giza, Egypten_US
dc.identifier.citationElsayed Alshaimaa M., Hegazy Usama M., Hegazy Marwa G. A., Abdel-Ghany Somia S., Salama Walaa H., Salem Ahmed M. H., Fahmy Afaf S.. Decolorization of Synthetic Dyes by Ficus carica Latex Peroxidase Isoenzymes. Biotechnology Journal International. 2018 Jul; 21(3): 1-14en_US
dc.identifier.issn2456-7051
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/188608
dc.languageenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.issuenumber3en_US
dc.relation.volume21en_US
dc.source.urihttps://doi.org/10.9734/BJI/2018/42682en_US
dc.subjectFicus caricaen_US
dc.subjectlatexen_US
dc.subjectperoxidaseen_US
dc.subjectdyes decolorizationen_US
dc.titleDecolorization of Synthetic Dyes by Ficus carica Latex Peroxidase Isoenzymesen_US
dc.typeJournal Articleen_US
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