Regeneration of encapsulated protocorms of Phaius tankervilliae stored at low temperature.

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dc.contributor.authorMalemnganba, Hen_US
dc.contributor.authorRay, B Ken_US
dc.contributor.authorBhattacharyya, Sen_US
dc.contributor.authorDeka, P Cen_US
dc.date.accessioned2009-05-28T10:51:14Z
dc.date.available2009-05-28T10:51:14Z
dc.date.issued1996-08-01en_US
dc.description.abstractEmbryos (80 days old) developed after selfing P. tankervilliae were cultured on Nitsch medium for protocorm development. Protocorms (70 days old) thus developed were encapsulated with alginate matrix. Ninety six per cent of freshly prepared encapsulated protocorms differentiated into shoots and roots when cultured on Nitsch medium. Storage of encapsulated protocorms in sealed petri plates or by embedding in liquid paraffin at 4 degrees C showed no reduction in their regeneration frequency up to 120 days when cultured on Nitsch medium. However, 90% of encapsulated protocorms stored at room temperature in empty petri plates differentiated within 35-40 days. Regeneration frequency of encapsulated protocorms was drastically reduced when stored in liquid paraffin at room temperature.en_US
dc.description.affiliationDepartment of Agricultural Biotechnology, Assam Agricultural, University, Jorha, India.en_US
dc.identifier.citationMalemnganba H, Ray BK, Bhattacharyya S, Deka PC. Regeneration of encapsulated protocorms of Phaius tankervilliae stored at low temperature. Indian Journal of Experimental Biology. 1996 Aug; 34(8): 802-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/55891
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAlginatesen_US
dc.subject.meshCryopreservationen_US
dc.subject.meshGlucuronic Aciden_US
dc.subject.meshHexuronic Acidsen_US
dc.subject.meshPlant Physiological Phenomenaen_US
dc.subject.meshPlants --embryologyen_US
dc.subject.meshRegeneration --physiologyen_US
dc.subject.meshSeedsen_US
dc.titleRegeneration of encapsulated protocorms of Phaius tankervilliae stored at low temperature.en_US
dc.typeJournal Articleen_US
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