Genetic instability in cervical cancer detected by arbitrarily primed polymerase chain reaction.

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dc.contributor.authorPaditaporn, Rujineeen_US
dc.contributor.authorRiengrojpitak, Sudaen_US
dc.contributor.authorPunyarit, Phaibulen_US
dc.contributor.authorChindavijak, Sangdeanen_US
dc.contributor.authorKaralak, Ananten_US
dc.contributor.authorPetmitr, Songsaken_US
dc.date.accessioned2009-05-27T17:46:14Z
dc.date.available2009-05-27T17:46:14Z
dc.date.issued2007-01-05en_US
dc.descriptionAsian Pacific Journal of Cancer Prevention.en_US
dc.description.abstractThe genetic instability in 54 Thai cervical cancer tissues were analyzed by Arbitrarily Primed Polymerase Chain Reaction (AP-PCR). The band alterations produced from 54 arbitrary primers were compared between the DNA finger printing from the patients and their corresponding normal cervical tissues. Results revealed 7 arbitrary primers provided DNA alteration patterns. Of these, an allelic loss in tumor DNA was found in DNA fingerprinting obtained from primers F-2 (64.8%), F-11 (68.5%), U-8 (51.9%), AE-3 (75.9%), AE-11 (53.7%), respectively. Moreover, DNA amplification was exhibited in patterns with primers B-12 (42.6%), J-16 (24.1%) and U-8 (70.4%). When genetic instability was investigated for associations with clinicopathological features, only the DNA amplified fragment with primer U-8 was significantly associated with stage II (P=0.030). Likewise, allelic loss amplified from arbitrary primer AE-3 showed significantly associate with age lower than 50 years old (P=0.003). Our findings suggest that the DNA alteration fragments produced from arbitrary primers of U-8 and AE-11 might be relevant to the pathogenesis of cervical cancer in Thai patients.en_US
dc.description.affiliationDepartment of Pathobiology, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.en_US
dc.identifier.citationPaditaporn R, Riengrojpitak S, Punyarit P, Chindavijak S, Karalak A, Petmitr S. Genetic instability in cervical cancer detected by arbitrarily primed polymerase chain reaction. Asian Pacific Journal of Cancer Prevention. 2007 Jan-Mar; 8(1): 109-12en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/37852
dc.language.isoengen_US
dc.source.urihttps://www.apocp.orgen_US
dc.subject.meshAdenocarcinoma --epidemiologyen_US
dc.subject.meshCarcinoma, Squamous Cell --epidemiologyen_US
dc.subject.meshDNA Fingerprintingen_US
dc.subject.meshDNA, Neoplasm --geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshGene Deletionen_US
dc.subject.meshGenetic Markersen_US
dc.subject.meshGenetic Predisposition to Diseaseen_US
dc.subject.meshHumansen_US
dc.subject.meshMutation --geneticsen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshThailand --epidemiologyen_US
dc.subject.meshUterine Cervical Neoplasms --epidemiologyen_US
dc.titleGenetic instability in cervical cancer detected by arbitrarily primed polymerase chain reaction.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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