Role of line probe assay in diagnosis and detection of drug resistance in Mycobacterium tuberculosis

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dc.contributor.authorMalhotra, Phalgunien_US
dc.contributor.authorChhina, Deepinderen_US
dc.contributor.authorGupta, Veenuen_US
dc.contributor.authorSingh, Akashdeepen_US
dc.contributor.authorSandhu, Diljoten_US
dc.date.accessioned2020-09-24T07:34:32Z
dc.date.available2020-09-24T07:34:32Z
dc.date.issued2019-04
dc.description.abstractTuberculosis caused by Mycobacterium tuberculosis has remained a major global health problem worldwide. TB requires prolonged period of time for isolation by conventional culture methods. The emergence and spread of multi drug resistant (MDR-TB) poses great threats and challenges in controlling the infection. MDR-TB is resistant to both first line drugs rifampicin and isoniazid. PCR tests are based on targeting the mutation in rpoB, katG and inhA genes which can detect resistance to these drugs. To compare microscopy, conventional culture and Line probe assay for the detection of M. tuberculosis & detect rifampicin and isoniazid resistance using Lineprobe assay in various clinical samples. A total of 347 suspected patients of tuberculosis were included in the study. Demographic details & clinical presentation was noted. Various samples were received & processed for ZN staining, culture on LJ media and Line probe assay. Out of 347 cases, majority of cases were in the age group of 51-60 years (18.4%). Majority of the population was males (65.1%). Among suspected tuberculosis patients, cough with expectoration (55.9%) was the commonest complaint. Microscopy was positive in 17.3%, conventional culture was positive in 16.1% and line probe assay was positive in 26.2%. Out of 347, 91 were diagnosed with MTB, out of which 85.7% were sensitive to both rifampicin and isoniazid whereas 14.3% showed resistance to either rifampicin / isoniazid or both. LPA & direct microscopy are a good screening method for early diagnosis and detection of drug resistance but are not a complete replacement of conventional culture which is still a gold standard.en_US
dc.identifier.affiliationsDepartments of Microbiology, Dayanand Medical College and Hospital, Ludhiana, India*en_US
dc.identifier.citationMalhotra Phalguni, Chhina Deepinder, Gupta Veenu, Singh Akashdeep, Sandhu Diljot. Role of line probe assay in diagnosis and detection of drug resistance in Mycobacterium tuberculosis. International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL). 2019 Apr; 8(2): 46-49en_US
dc.identifier.issn2282-0238
dc.identifier.issn2393-932X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/203838
dc.languageenen_US
dc.publisherSociety for research and Development in Educationen_US
dc.relation.issuenumber2en_US
dc.relation.volume8en_US
dc.source.urihttps://doi.org/10.21276/IJRDPL.2278-0238.2019.8(2).46-49en_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectMDR-TBen_US
dc.subjectLine probe assayen_US
dc.subjectRifampicinen_US
dc.subjectIsoniaziden_US
dc.titleRole of line probe assay in diagnosis and detection of drug resistance in Mycobacterium tuberculosisen_US
dc.typeJournal Articleen_US
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