Inhibition of CYP3A4 and CYP2C9 by podophyllotoxin: Implication for clinical drug–drug interactions.

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dc.contributor.authorSong, Jin-Hui
dc.contributor.authorSun, Dong-Xue
dc.contributor.authorChen, Bin
dc.contributor.authorJi, Dai-Hong
dc.contributor.authorPu, Jie
dc.contributor.authorJie, Xu
dc.contributor.authorTian, Feng-De
dc.contributor.authorGuo, Lin
dc.date.accessioned2015-08-08T09:55:19Z
dc.date.available2015-08-08T09:55:19Z
dc.date.issued2011-12
dc.description.abstractPodophyllotoxin (PPT) and its derivatives exert significant anti-cancer activities, and one derivative etoposide is often utilized to treat various cancers in the clinic. The aim of the present study is to investigate the inhibitory effects of PPT on major cytochrome P450 (CYP) isoforms in human livers. Inhibition of CYP3A4, CYP2C9, CYP2C8, CYP2D6, CYP2E1 and CYP2A6 by PPT was investigated in the human liver microsomal system. Time-dependent inhibition of CYP3A4 by PPT was also evaluated. The results showed that PPT strongly exhibited inhibitory effects on CYP3A4 and CYP2C9 in a concentration-dependent manner. Half inhibition concentration (IC50) was 1.1±0.3 and 4.6±0.3 μM for CYP3A4 and CYP2C9, respectively. Inhibition kinetic analysis showed that PPT exhibited competitive inhibition towards CYP3A4 and CYP2C9 with Ki of 1.6 and 2.0 μM, respectively. Additionally, PPT exerted time-dependent inhibition towards CYP3A4 and the kinetic parameters were 4.4±2.1 μM and 0.06±0.01 min–1 for KI and kinact, respectively. Our experimental data indicate that potential drug–drug interaction (DDI) might exist when PPT is co-administered with the substrates which mainly undergo CYP3A4- or CYP2C9-mediated metabolism.en_US
dc.identifier.citationSong Jin-Hui, Sun Dong-Xue, Chen Bin, Ji Dai-Hong, Pu Jie, Jie Xu, Tian Feng-De, Guo Lin. Inhibition of CYP3A4 and CYP2C9 by podophyllotoxin: Implication for clinical drug–drug interactions. Journal of Biosciences. 2011 Dec; 36 (5): 879-895.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/161622
dc.language.isoenen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/dec2011/879.pdfen_US
dc.subjectCytochrome P450 (CYP)en_US
dc.subjectdrug–drug interaction (DDI)en_US
dc.subjectpodophyllotoxin (PPT)en_US
dc.subjecttime-dependent inhibition (TDI)en_US
dc.titleInhibition of CYP3A4 and CYP2C9 by podophyllotoxin: Implication for clinical drug–drug interactions.en_US
dc.typeArticleen_US
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