Evaluation of arjunolic acid against Brucella melitenis and in vitro cytotoxic study of lung adenocarcinomic cell line (A549)

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dc.contributor.authorYasasve, Men_US
dc.contributor.authorKumaran, P Muthuen_US
dc.contributor.authorManoj, Den_US
dc.contributor.authorKanmani, Men_US
dc.contributor.authorRamesh, A Saien_US
dc.date.accessioned2023-08-09T06:20:55Z
dc.date.available2023-08-09T06:20:55Z
dc.date.issued2022-07
dc.description.abstractBrucellosis, a neglected tropical disease of zoonotic nature, is caused by the genus Brucella, specifically by Brucella abortus and B. melitensis in cattle and humans, respectively. Arjunolic acid (AA) is a triterpenoid, isolated from Terminalia arjuna (Roxb.) Wight & Arn., a medicinally important plant used to treat various diseases in the Indian system of medicine. Here, we tried to evaluate AA for its antibacterial activity on Brucella and the in vitro cytotoxicity assay on human lung adenocarcinomic alveolar basal epithelial cell line (A549). Also, we assessed the synergistic effect of arjunolic acid and Tarenna asiatica (L.) Kuntze ex K.Schum. on B. melitensis. AA displayed a considerable antibacterial activity [zone of inhibition (9 mm) with a minimum inhibitory concentration of 30 ?g/mL] against B. melitensis. The rate of cell death for the cancer cells were at 100 ?g/mL concentration of AA was 82% which indicates that AA shows significant membrane disruption to cancer cells. The estimated IC50 of AA against the A549 cell line was 139.90 ?g/mL. The highest synergistic activity was exhibited forming a zone of inhibition measuring 10mm when arjunolic acid and AqE of T. asiatica was added in the concentration of 1:1, respectively.en_US
dc.identifier.affiliationsDepartment of Biotechnology, Sree Sastha Institute of Engineering and Technology (Affiliated to Anna University), Chennai - 600 123, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Biotechnology, Vel Tech High Tech Dr. Rangarajan Dr.Sakunthala Engineering College (An Autonomous Institution), Chennai - 600 062, Tamil Nadu, Indiaen_US
dc.identifier.citationYasasve M, Kumaran P Muthu, Manoj D, Kanmani M, Ramesh A Sai. Evaluation of arjunolic acid against Brucella melitenis and in vitro cytotoxic study of lung adenocarcinomic cell line (A549). Indian Journal of Experimental Biology. 2022 Jul; 60(7): 510-513en_US
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/222569
dc.languageenen_US
dc.publisherCSIR-NIScPRen_US
dc.relation.issuenumber7en_US
dc.relation.volume60en_US
dc.source.urihttps://doi.org/10.56042/ijeb.v60i07.64073en_US
dc.subjectAdenocarcinomaen_US
dc.subjectAnticancer activityen_US
dc.subjectArjuna treeen_US
dc.subjectAntibacterial activityen_US
dc.subjectBrucellosisen_US
dc.subjectLung cancer cell lineen_US
dc.subjectTarenna asiaticaen_US
dc.subjectTerminalia arjunaen_US
dc.titleEvaluation of arjunolic acid against Brucella melitenis and in vitro cytotoxic study of lung adenocarcinomic cell line (A549)en_US
dc.typeJournal Articleen_US
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