Influence of environmental hypertonicity on the induction of ureogenesis and amino acid metabolism in air-breathing walking catfish (Clarias batrachus, Bloch).

Simple item page
dc.contributor.authorBanerjee, Bodhisattwa
dc.contributor.authorBhuyan, Gitalee
dc.contributor.authorSaha, Nirmalendu
dc.date.accessioned2014-12-11T09:00:34Z
dc.date.available2014-12-11T09:00:34Z
dc.date.issued2014-07
dc.description.abstractEffect of environmental hypertonicity, due to exposure to 300 mM mannitol solution for 7 days, on the induction of ureogenesis and also on amino acid metabolism was studied in the air-breathing walking catfish, C. batrachus, which is already known to have the capacity to face the problem of osmolarity stress in addition to other environmental stresses in its natural habitats. Exposure to hypertonic mannitol solution led to reduction of ammonia excretion rate by about 2-fold with a concomitant increase of urea-N excretion rate by about 2-fold. This was accompanied by significant increase in the levels of both ammonia and urea in different tissues and also in plasma. Further, the environmental hypertonicity also led to significant accumulation of different non-essential free amino acids (FAAs) and to some extent the essential FAAs, thereby causing a total increase of non-essential FAA pool by 2-3-fold and essential FAA pool by 1.5-2.0-fold in most of the tissues studied including the plasma. The activities of three ornithine-urea cycle (OUC) enzymes such as carbamoyl phosphate synthetase, argininosuccinate synthetase and argininosuccinate lyase in liver and kidney tissues, and four key amino acid metabolism-related enzymes such as glutamine synthetase, glutamate dehydrogenase (reductive amination), alanine aminotransaminase and aspartate aminotransaminase were also significantly up-regulated in different tissues of the fish while exposing to hypertonic environment. Thus, more accumulation and excretion of urea-N observed during hypertonic exposure were probably associated with the induction of ureogenesis through the induced OUC, and the increase of amino acid pool was probably mainly associated with the up-regulation of amino acid synthesizing machineries in this catfish in hypertonic environment. These might have helped the walking catfish in defending the osmotic stress and to acclimatize better under hypertonic environment, which is very much uncommon among freshwater teleosts.en_US
dc.identifier.citationBanerjee Bodhisattwa, Bhuyan Gitalee, Saha Nirmalendu. Influence of environmental hypertonicity on the induction of ureogenesis and amino acid metabolism in air-breathing walking catfish (Clarias batrachus, Bloch). Indian Journal of Experimental Biology. 2014 Jul; 52(7): 728-738.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/153753
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/29046en_US
dc.subjectAmino acid metabolismen_US
dc.subjectAmmoniaen_US
dc.subjectClarias batrachusen_US
dc.subjectEnvironmental hypertonicityen_US
dc.subjectMannitolen_US
dc.subjectOrnithine-urea cycleen_US
dc.subjectUreaen_US
dc.subjectWalking catfishen_US
dc.subject.meshAir
dc.subject.meshAmino Acids --metabolism
dc.subject.meshAmmonia --analysis
dc.subject.meshAnimals
dc.subject.meshCatfishes --growth & development
dc.subject.meshCatfishes --metabolism
dc.subject.meshDiuretics, Osmotic --pharmacology
dc.subject.meshEnvironment
dc.subject.meshHypertonic Solutions --pharmacology
dc.subject.meshMannitol --pharmacology
dc.subject.meshOrnithine --metabolism
dc.subject.meshOsmosis --drug effects
dc.subject.meshRespiration
dc.subject.meshUrea --analysis
dc.subject.meshUrea --metabolism
dc.subject.meshWalking
dc.titleInfluence of environmental hypertonicity on the induction of ureogenesis and amino acid metabolism in air-breathing walking catfish (Clarias batrachus, Bloch).en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2014v52n7p728.pdf
Size:
248.64 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology