Production of a cellulase-free alkaline xylanase from Bacillus pumilus MTCC 5015 by submerged fermentation and its application in biobleaching.

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dc.contributor.authorThomas, Leya
dc.contributor.authorSindhu, Raveendran
dc.contributor.authorBinod, Parameswaran
dc.contributor.authorPandey, Ashok
dc.date.accessioned2015-06-27T07:34:53Z
dc.date.available2015-06-27T07:34:53Z
dc.date.issued2015-06
dc.description.abstractHere, we described the production of a cellulase-free alkaline xylanase from Bacillus pumilus MTCC 5015 by submerged fermentation and its application in biobleaching. Various process parameters affecting xylanase production by B. pumilus were optimized by adopting a Plackett-Burman design (PBD) as well as Response surface methodology (RSM). These statistical methods aid in improving the enzyme yield by analysing the individual crucial components of the medium. Maximum production was obtained with 4% yeast extract, 0.08% magnesium sulphate, 30 h of inoculum age, incubation temperature of 33.5 °C and pH 9.0. Under optimized conditions, the xylanase activity was 372 IU/ml. Media engineering improved a 5-fold increase in the enzyme production. Scanning electron microscopy (SEM) showed significant changes on the surface of xylanase treated pulps as a result of xylan hydrolysis. Increased roughness of paper carton fibres was apparent in scanning electron micrograph due to opening of the micro fibrils present on the surface by xylanase action. The untreated pulp did not show any such change. These results demonstrated that the B. pumilus MTCC 5015 xylanase was effective in bio-bleaching of paper carton.en_US
dc.identifier.citationThomas Leya, Sindhu Raveendran, Binod Parameswaran, Pandey Ashok. Production of a cellulase-free alkaline xylanase from Bacillus pumilus MTCC 5015 by submerged fermentation and its application in biobleaching. Indian Journal of Experimental Biology. 2015 Jun; 53(6): 356-363.en_US
dc.identifier.issn0975-1009
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158505
dc.language.isoenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/31576en_US
dc.subjectPaper cartonen_US
dc.subjectResponse surface methodology (RSM)en_US
dc.subjectXylanen_US
dc.subject.meshBacillus --chemistry
dc.subject.meshBacillus --classification
dc.subject.meshBiotechnology
dc.subject.meshCellulose --metabolism
dc.subject.meshFermentation
dc.subject.meshPaper
dc.subject.meshXylosidases --biosynthesis
dc.subject.meshXylosidases --chemical synthesis
dc.titleProduction of a cellulase-free alkaline xylanase from Bacillus pumilus MTCC 5015 by submerged fermentation and its application in biobleaching.en_US
dc.typeArticleen_US
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