Indole acetic acid production by fluorescent Pseudomonas spp. from the rhizosphere of Plectranthus amboinicus (Lour.) Spreng. and their variation in extragenic repetitive DNA sequences.

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dc.contributor.authorSethia, Bedhya
dc.contributor.authorMustafa, Mariam
dc.contributor.authorManohar, Sneha
dc.contributor.authorPatil, Savita V
dc.contributor.authorJayamohan, Nellickal Subramanian
dc.contributor.authorKumudini, Belur Satyan
dc.date.accessioned2015-06-27T07:21:33Z
dc.date.available2015-06-27T07:21:33Z
dc.date.issued2015-06
dc.description.abstractFluorescent Pseudomonas (FP) is a heterogenous group of growth promoting rhizobacteria that regulate plant growth by releasing secondary metabolic compounds viz., indole acetic acid (IAA), siderophores, ammonia and hydrogen cyanide. In the present study, IAA producing FPs from the rhizosphere of Plectranthus amboinicus were characterized morphologically, biochemically and at the molecular level. Molecular identification of the isolates were carried out using Pseudomonas specific primers. The effect of varying time (24, 48, 72 and 96 h), Trp concentrations (100, 200, 300, 400 and 500 µg.ml-1), temperature (10, 26, 37 and 50±2 °C) and pH (6, 7 and 8) on IAA production by 10 best isolates were studied. Results showed higher IAA production at 72 h incubation, at 300 µg.ml-1 Trp concentration, temperature 26±2 °C and pH 7. TLC with acidified ethyl acetate extract showed that the IAA produced has a similar Rf value to that of the standard IAA. Results of TLC were confirmed by HPLC analysis. Genetic diversity of the isolates was also studied using 40 RAPD and 4 Rep primers. Genetic diversity parameters such as dominance, Shannon index and Simpson index were calculated. Out of 40 RAPD primers tested, 9 (2 OP-D series and 7 OP-E series) were shortlisted for further analysis. Studies using RAPD, ERIC, BOX, REP and GTG5 primers revealed that isolates exhibit significant diversity in repetitive DNA sequences irrespective of the rhizosphere.en_US
dc.identifier.citationSethia Bedhya, Mustafa Mariam, Manohar Sneha, Patil Savita V, Jayamohan Nellickal Subramanian, Kumudini Belur Satyan. Indole acetic acid production by fluorescent Pseudomonas spp. from the rhizosphere of Plectranthus amboinicus (Lour.) Spreng. and their variation in extragenic repetitive DNA sequences. Indian Journal of Experimental Biology. 2015 Jun; 53(6): 342-349.en_US
dc.identifier.issn0975-1009
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/158501
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/31574en_US
dc.subjectIAAen_US
dc.subjectIndian borageen_US
dc.subjectNatural auxinen_US
dc.subjectPGPRen_US
dc.subjectRep PCRen_US
dc.subjectRepetitive DNA sequencesen_US
dc.subjectRhizosphere effecten_US
dc.subject.meshFluorescence
dc.subject.meshBase Sequence --genetics
dc.subject.meshIndoleacetic Acids --biosynthesis
dc.subject.meshPlectranthus --classification
dc.subject.meshPlectranthus --metabolism
dc.subject.meshPolymerase Chain Reaction --methods
dc.subject.meshPseudomonas --classification
dc.subject.meshPseudomonas --metabolism
dc.subject.meshRhizosphere
dc.titleIndole acetic acid production by fluorescent Pseudomonas spp. from the rhizosphere of Plectranthus amboinicus (Lour.) Spreng. and their variation in extragenic repetitive DNA sequences.en_US
dc.typeArticleen_US
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