Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand.

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dc.contributor.authorPandey, B Den_US
dc.contributor.authorKarabatsos, Nen_US
dc.contributor.authorCropp, Ben_US
dc.contributor.authorTagaki, Men_US
dc.contributor.authorTsuda, Yen_US
dc.contributor.authorIchinose, Aen_US
dc.contributor.authorIgarashi, Aen_US
dc.date.accessioned2009-05-27T16:42:03Z
dc.date.available2009-05-27T16:42:03Z
dc.date.issued1999-03-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractA virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.en_US
dc.description.affiliationDepartment of Virology, Institute for Tropical Medicine, Nagasaki University, Nagasaki City, Japan. b-pandey@alpha.med.nagasaki-u.ac.jpen_US
dc.identifier.citationPandey BD, Karabatsos N, Cropp B, Tagaki M, Tsuda Y, Ichinose A, Igarashi A. Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 1999 Mar; 30(1): 161-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35812
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/1999-30-1/1999-30-1-161.pdfen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCulex --virologyen_US
dc.subject.meshDNA, Viral --analysisen_US
dc.subject.meshFlavivirus --classificationen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshNeutralization Testsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSerotypingen_US
dc.subject.meshThailanden_US
dc.subject.meshVirologyen_US
dc.subject.meshVirus Cultivationen_US
dc.titleIdentification of a flavivirus isolated from mosquitos in Chiang Mai Thailand.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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