Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand.
dc.contributor.author | Pandey, B D | en_US |
dc.contributor.author | Karabatsos, N | en_US |
dc.contributor.author | Cropp, B | en_US |
dc.contributor.author | Tagaki, M | en_US |
dc.contributor.author | Tsuda, Y | en_US |
dc.contributor.author | Ichinose, A | en_US |
dc.contributor.author | Igarashi, A | en_US |
dc.date.accessioned | 2009-05-27T16:42:03Z | |
dc.date.available | 2009-05-27T16:42:03Z | |
dc.date.issued | 1999-03-01 | en_US |
dc.description | The Southeast Asian Journal of Tropical Medicine and Public Health. | en_US |
dc.description.abstract | A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus. | en_US |
dc.description.affiliation | Department of Virology, Institute for Tropical Medicine, Nagasaki University, Nagasaki City, Japan. b-pandey@alpha.med.nagasaki-u.ac.jp | en_US |
dc.identifier.citation | Pandey BD, Karabatsos N, Cropp B, Tagaki M, Tsuda Y, Ichinose A, Igarashi A. Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 1999 Mar; 30(1): 161-5 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/35812 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.tm.mahidol.ac.th/seameo/1999-30-1/1999-30-1-161.pdf | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Culex --virology | en_US |
dc.subject.mesh | DNA, Viral --analysis | en_US |
dc.subject.mesh | Flavivirus --classification | en_US |
dc.subject.mesh | Microscopy, Electron | en_US |
dc.subject.mesh | Neutralization Tests | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Serotyping | en_US |
dc.subject.mesh | Thailand | en_US |
dc.subject.mesh | Virology | en_US |
dc.subject.mesh | Virus Cultivation | en_US |
dc.title | Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand. | en_US |
dc.type | Journal Article | en_US |
dc.type | Research Support, Non-U.S. Gov't | en_US |