Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand.
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Date
1999-03-01
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Abstract
A virus isolate, ThCAr105/92, from a pool of mosquitos, Culex tritaeniorhynchus, collected in Chiang Mai, Thailand in 1992, appeared to be a member of the genus Flavivirus of the family Flaviviridae, based on the reverse transcription polymerase chain reaction (RT-PCR) using flavivirus cross-reacting primer pairs, electron microscopic examination, and serological tests. However, RT-PCR using Japanese encephalitis (JE) virus-specific primers showed that the isolate was different from JE virus. Sucrose density gradient sedimentation of the virus replicated in C6/36 cells indicated that the virus is relatively unstable in the infected culture fluids at 37 degrees C. Antibody prepared against this virus and a virus seed for the isolate were tested by cross neutralization against a panel of flaviviruses and the results showed that the new isolate was a distinct subtype of Tembusu virus.
Description
The Southeast Asian Journal of Tropical Medicine and Public Health.
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Citation
Pandey BD, Karabatsos N, Cropp B, Tagaki M, Tsuda Y, Ichinose A, Igarashi A. Identification of a flavivirus isolated from mosquitos in Chiang Mai Thailand. The Southeast Asian Journal of Tropical Medicine and Public Health. 1999 Mar; 30(1): 161-5