Determination of the activity and kinetic parameters of polyphenol oxidase enzyme in crude extracts of some Jordanian plants

Simple item page
dc.contributor.authorAtrooz, Omar Mohammaden_US
dc.contributor.authorAlKhamaisa, Nooh Khairen_US
dc.contributor.authorAlRawashdeh, Ibrahim M.en_US
dc.date.accessioned2020-10-16T08:50:37Z
dc.date.available2020-10-16T08:50:37Z
dc.date.issued2020-07
dc.description.abstractThe activity and kinetic parameters of the enzyme polyphenol oxidase (PPO) in crude extracts of the fruits ofParadise apple (Pyrus malus L.), cucumber (Cucumis sativus L.), squash (Cucurbita pepo L.), pomegranaterind and seeds (Punica granatum L.), and the leaf of cactus (Opuntia ficus-indica (L.) were investigated. Thehighest browning intensity was found in P. apple, followed by pomegranate seeds and cucumber fruits. Theoptimum activity of the enzyme was shown at pH 7.0 for P. apple and cucurbita, but it was found at pH 6.0 forthe rind and seeds of the pomegranate. The PPO of cactus leaf and cucumber fruits showed two peaks at pH 4.0and 7.0. On the other hand, the optimal temperature found for PPO activity in these plants was around 40°C.A strong correlation with value (R) = 0.9485 between the browning intensity and PPO activity in all studiedsamples was obtained.en_US
dc.identifier.affiliationsDepartment of Biological Science, Faculty of Science, Mutah University, Mutah, Jordanen_US
dc.identifier.affiliationsMinistry of Education, Hashemite Kingdom of Jordan, Amman, Jordanen_US
dc.identifier.affiliationsDepartment of Biological Science, Faculty of Science, Al-Hussein Bin Talal University, Ma'an, Jordanen_US
dc.identifier.citationAtrooz Omar Mohammad, AlKhamaisa Nooh Khair, AlRawashdeh Ibrahim M.. Determination of the activity and kinetic parameters of polyphenol oxidase enzyme in crude extracts of some Jordanian plants. Journal of Applied Biology & Biotechnology. 2020 Jul; 2020 Jul: 69-74en_US
dc.identifier.issn2455-7005
dc.identifier.issn2347-212X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/209924
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber4en_US
dc.relation.volume8en_US
dc.source.urihttps://dx.doi.org//10.7324/JABB.2020.80411en_US
dc.subjectPolyphenol oxidaseen_US
dc.subjectkineticsen_US
dc.subjectbrowningen_US
dc.subjectrelative activityen_US
dc.subjectactivation energy.en_US
dc.titleDetermination of the activity and kinetic parameters of polyphenol oxidase enzyme in crude extracts of some Jordanian plantsen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jabb2020v8n4p69.pdf
Size:
592.63 KB
Format:
Adobe Portable Document Format
Download
Collections
Journal of Applied Biology & Biotechnology