Enzyme Functional Screening, Discovery and Engineering; Automation, Metagenomics and High-throughput Approaches

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dc.contributor.authorAli, Sikanderen_US
dc.contributor.authorHussain, Syed Shahiden_US
dc.date.accessioned2020-05-05T07:04:30Z
dc.date.available2020-05-05T07:04:30Z
dc.date.issued2020-01
dc.description.abstractConcerned with the construction and design of novel biocatalysts, the enzyme engineering served to overcome the limitations of native enzymes, in order to create biocatalysts with tailored functions, to facilitate industrial applications. The enzymes, being recognized by screening and discovery workflows and further tailored by engineering platforms, are of immense potential as improved biocatalysts. Functional metagenomics is a powerful tool to identify novel enzymes followed by the construction of metagenome-based enzyme libraries. And the subsequent screening of these enzyme libraries is in turn facilitated by ultra-high-throughput-based, for example FACS or microfluidics, enzyme engineering technologies. Relies on the compartmentalization of reaction components, in order to detect and measure assay signal within the reaction compartments, the enzyme engineering platforms are designed which include cell-as-compartment platforms, droplet-based platforms andmicro-chamber-based platforms. The metagenomics approach and high-throughput screening by these three prime enzyme engineer platforms are the focus of this review.en_US
dc.identifier.affiliationsInstitute of Industrial Biotechnology, GC University, Lahore, Pakistanen_US
dc.identifier.citationAli Sikander, Hussain Syed Shahid. Enzyme Functional Screening, Discovery and Engineering; Automation, Metagenomics and High-throughput Approaches . International Journal of Biochemistry Research & Review. 2020 Jan; 28(4): 1-12en_US
dc.identifier.issn2231-086X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/200765
dc.languageenen_US
dc.publisherScience Domain Internationalen_US
dc.relation.issuenumber4en_US
dc.relation.volume28en_US
dc.source.urihttps://doi.org/10.9734/IJBCRR/2019/v28i430156en_US
dc.subjectBiocatalysts;compartmentalization;FASC;microfluidics;microchamberen_US
dc.titleEnzyme Functional Screening, Discovery and Engineering; Automation, Metagenomics and High-throughput Approachesen_US
dc.typeJournal Articleen_US
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