Isolation of Mycobacterium tuberculosis protein antigens ES-3 1, ES-43 and EST-6 of diagnostic interest from tubercle bacilli by affinity chromatography.

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dc.contributor.authorUpadhye, Vijayen_US
dc.contributor.authorSaha-Roy, Santaen_US
dc.contributor.authorShende, Nirajen_US
dc.contributor.authorKumar, Satishen_US
dc.contributor.authorHarinath, B Cen_US
dc.date.accessioned2009-05-28T14:08:15Z
dc.date.available2009-05-28T14:08:15Z
dc.date.issued2007-07-08en_US
dc.description.abstractImmunodiagnostically useful M. tuberculosis H37Ra protein antigens ES-31, ES-43 and EST-6 were isolated from detergent soluble sonicate (DSS) antigen using monospecific antibodies by affinity chromatography and compared with similar antigens isolated from M. tuberculosis culture filtrate for seroreactivity in tuberculosis sera by Indirect Enzyme Linked Immunosorbent Assay. Recovery of affinity purified ES-31, ES-43 and EST-6 antigen from DSS antigen was approximately 3, 3.5 and 4% respectively, compared to 10, 9 and 6.3% from culture filtrate. Affinity purified ES-31, ES-43 and EST-6 antigens from both culture filtrate as well as DSS antigen showed similar seroreactivity with overall sensitivity 85, 80 and 75% respectively and specificity of 85% at optimum concentration of 50 pg protein of each antigen. The results suggest that DSS antigen may be a promising antigen source for isolating antigens of diagnostic interest obviating the need for cumbersome, time-consuming culture techniques of mycobacteria.en_US
dc.description.affiliationJamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha 442 102, India.en_US
dc.identifier.citationUpadhye V, Saha-Roy S, Shende N, Kumar S, Harinath BC. Isolation of Mycobacterium tuberculosis protein antigens ES-3 1, ES-43 and EST-6 of diagnostic interest from tubercle bacilli by affinity chromatography. Indian Journal of Experimental Biology. 2007 Jul; 45(7): 599-602en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60318
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/5329/1/IJEB%2045%287%29%20599-602.pdfen_US
dc.subject.meshAntigens, Bacterial --chemistryen_US
dc.subject.meshBacterial Proteins --chemistryen_US
dc.subject.meshChromatography, Affinity --methodsen_US
dc.subject.meshDetergents --pharmacologyen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --instrumentationen_US
dc.subject.meshExpressed Sequence Tagsen_US
dc.subject.meshHumansen_US
dc.subject.meshMycobacterium tuberculosis --metabolismen_US
dc.subject.meshTuberculosis, Pulmonary --microbiologyen_US
dc.titleIsolation of Mycobacterium tuberculosis protein antigens ES-3 1, ES-43 and EST-6 of diagnostic interest from tubercle bacilli by affinity chromatography.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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