Generation of HIV-1 based bi-cistronic lentiviral vectors for stable gene expression and live cell imaging.

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dc.contributor.authorSehgal, Lalit
dc.contributor.authorBudnar, Srikanth
dc.contributor.authorBhatt, Khyati
dc.contributor.authorSansare, Sneha
dc.contributor.authorMukhopadhaya, Amitabha
dc.contributor.authorKalraiya, Rajiv D
dc.contributor.authorDalal, Sorab N
dc.date.accessioned2013-02-27T06:35:04Z
dc.date.available2013-02-27T06:35:04Z
dc.date.issued2012-10
dc.description.abstractThe study of protein-protein interactions, protein localization, protein organization into higher order structures and organelle dynamics in live cells, has greatly enhanced the understanding of various cellular processes. Live cell imaging experiments employ plasmid or viral vectors to express the protein/proteins of interest fused to a fluorescent protein. Unlike plasmid vectors, lentiviral vectors can be introduced into both dividing and non dividing cells, can be pseudotyped to infect a broad or narrow range of cells, and can be used to generate transgenic animals. However, the currently available lentiviral vectors are limited by the choice of fluorescent protein tag, choice of restriction enzyme sites in the Multiple Cloning Sites (MCS) and promoter choice for gene expression. In this report, HIV-1 based bi-cistronic lentiviral vectors have been generated that drive the expression of multiple fluorescent tags (EGFP, mCherry, ECFP, EYFP and dsRed), using two different promoters. The presence of a unique MCS with multiple restriction sites allows the generation of fusion proteins with the fluorescent tag of choice, allowing analysis of multiple fusion proteins in live cell imaging experiments. These novel lentiviral vectors are improved delivery vehicles for gene transfer applications and are important tools for live cell imaging in vivo.en_US
dc.identifier.citationSehgal Lalit, Budnar Srikanth, Bhatt Khyati, Sansare Sneha, Mukhopadhaya Amitabha, Kalraiya Rajiv D, Dalal Sorab N. Generation of HIV-1 based bi-cistronic lentiviral vectors for stable gene expression and live cell imaging. Indian Journal of Experimental Biology. 2012 Oct; 50(10): 669-676.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/145302
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/14789en_US
dc.subjectBi-cistronic lentiviral vectoren_US
dc.subjectFRETen_US
dc.subjectFluorescent tagen_US
dc.subjectHIV-Ien_US
dc.subjectIn vivo imagingen_US
dc.subjectMultiple cloning siteen_US
dc.subjectStable transgene expressionen_US
dc.titleGeneration of HIV-1 based bi-cistronic lentiviral vectors for stable gene expression and live cell imaging.en_US
dc.typeArticleen_US
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