Effect of phosphocreatine on H+ extrusion, pHi and dimorphism in Candida albicans.

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dc.contributor.authorManzoor, Nikhaten_US
dc.contributor.authorAmin, Men_US
dc.contributor.authorKhan, Luqman Aen_US
dc.date.accessioned2009-05-28T16:21:44Z
dc.date.available2009-05-28T16:21:44Z
dc.date.issued2002-07-25en_US
dc.description.abstractCandida albicans is an opportunistic pathogen. Its proliferation in human hosts is believed to be controlled by immunologic mechanisms. The plasma membrane of the fungus possesses an H(+)-ATPase (PM-ATPase) which actively extrudes protons to generate an electrochemical gradient which is used in co-transport of nutrients. This ATPase is associated with the growth, dimorphism and pathogenicity of the fungus. The physiological concentration of phosphocreatine (PCr) is 20-35 mM in skeletal muscles. H(+)-extrusion in Candida cells was strongly inhibited by PCr; 44% at 20 mM and 69% at 40 mM. H(+)-extrusion was stimulated 6.2-fold in the presence of 10 mM glucose. This glucose stimulated extrusion was inhibited significantly by PCr; 36% at 20 mM and 53% at 40 mM. The intracellular pH pattern of cells destined to differentiate was greatly altered in the presence of PCr. Evagination time for control cells was between 90-120 min. PCr, delayed dimorphism, reduced the population of cells differentiating to hyphae and also reduced the length of hyphae after each time interval. Only 60% differentiation was observed with 10 mM PCr and 40% for higher PCr concentration even after 210 min. Direct interaction of PM-ATPase and PCr has been demonstrated by difference spectrum measurement employing stopped flow spectrophotometer. It can be concluded that PCr may be playing a significant role in checking growth and pathogenesis of C. albicans.en_US
dc.description.affiliationDepartment of Biosciences, Jamia Millia Islamia, New Delhi 110025, India.en_US
dc.identifier.citationManzoor N, Amin M, Khan LA. Effect of phosphocreatine on H+ extrusion, pHi and dimorphism in Candida albicans. Indian Journal of Experimental Biology. 2002 Jul; 40(7): 785-90en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/63138
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAdenosine Triphosphatases --metabolismen_US
dc.subject.meshCandida albicans --enzymologyen_US
dc.subject.meshHydrogen --metabolismen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshPhosphocreatine --pharmacologyen_US
dc.titleEffect of phosphocreatine on H+ extrusion, pHi and dimorphism in Candida albicans.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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