Identification and characterization of causative agents of brown leaf spot disease of cassava in Kenya

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dc.contributor.authorNg’ang’a, Perpetuar Wangarien_US
dc.contributor.authorMiano, Douglas Watukuen_US
dc.contributor.authorWagacha, John Mainaen_US
dc.contributor.authorPaul, Kuriaen_US
dc.date.accessioned2020-10-16T08:50:15Z
dc.date.available2020-10-16T08:50:15Z
dc.date.issued2019-11
dc.description.abstractThis study was conducted to investigate the causal agent of brown leaf spot (BLS) of cassava in Kenyanfields. Infected cassava leaf samples showing BLS disease symptoms were collected from Kenya Agriculturaland Livestock Research Organization (KALRO) experimental fields in central and western Kenya. Fungalpathogens associated with the disease were isolated from the cassava leaf samples on potato dextrose agaramended with antibiotics. Three fungal isolates, belonging to the genera Colletotrichum, Alternaria, andCladosporium, were identified as the causative agents of BLS with relative prevalence of 41%, 24%, and 18%,respectively. Susceptible cassava variety TME 204 was inoculated with single and combinations of purifiedisolates of the three pathogens in a randomized design in the greenhouse. The combination of the three isolatesresulted in typical BLS symptoms as observed in the field. The findings of this study would help to understandthe disease, contribute to its better management, and eventually alleviate food insecurity, especially in theregions where cassava is a major staple food and a source of income.en_US
dc.identifier.affiliationsSchool of Biological Sciences, University of Nairobi, 30197 Nairobi, Kenyaen_US
dc.identifier.affiliationsDepartment of Plant Science and Crop Protection, Faculty of Agriculture, University of Nairobi, 29053 00625 Kangemi, Kenyaen_US
dc.identifier.affiliationsSchool of Biological Sciences, University of Nairobi, 30197 Nairobi, Kenyaen_US
dc.identifier.affiliationsBiotechnology Research Institute, Kenya Agricultural and Livestock Research Organization, 57811-00200 Nairobi, Kenyen_US
dc.identifier.citationNg’ang’a Perpetuar Wangari, Miano Douglas Watuku, Wagacha John Maina, Paul Kuria. Identification and characterization of causative agents of brown leaf spot disease of cassava in Kenya. Journal of Applied Biology & Biotechnology. 2019 Nov; 2019 Nov: 1-7en_US
dc.identifier.issn2455-7005
dc.identifier.issn2347-212X
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/209851
dc.languageenen_US
dc.publisherOpen Science Publishers LLPen_US
dc.relation.issuenumber6en_US
dc.relation.volume7en_US
dc.source.urihttps://dx.doi.org//10.7324/JABB.2019.70601en_US
dc.subjectCassavaen_US
dc.subjectpathogenen_US
dc.subjectAlternariaen_US
dc.subjectColletotrichumen_US
dc.subjectCladosporiumen_US
dc.subjectsynergismen_US
dc.titleIdentification and characterization of causative agents of brown leaf spot disease of cassava in Kenyaen_US
dc.typeJournal Articleen_US
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