The Effects of Cosmos caudatus (Ulam Raja) on Detoxifying Enzymes in Extrahepatic Organs in Mice.
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Date
2015-01
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Abstract
‘Ulam Raja’ or Cosmos caudatus is a common appetizer (ulam) consumed by the Malay community in Malaysia.
However, in vivo studies pertaining to its antioxidant and chemoprotective properties are lacking. This study was
done to determine the effects of Cosmos caudatus on detoxifying enzymes in extrahepatic organs (lungs, kidneys
and stomach) in mice. Thirty adult male white mice were treated orally for 21 days with different doses of ‘Ulam
Raja’ aqueous extract (UR) (100, 500, 1000mg/kg). The control group was given normal saline by oral gavage.
Mice fed with diet containing 0.5% butylated hydroxyanisole (BHA) were used as positive control. After 21
days, the mice were sacrificed and extrahepatic organs were harvested. The activities of several detoxifying
enzymes [catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), DT-diaphorase (DTD)]
were measured. Lipid peroxidation level was determined by measuring malondialdehyde (MDA) concentration.
In lungs, 100, 500 & 1000 mg/kg UR oral supplementation resulted in significant increases in CAT, SOD and
GST activities. DTD activity in lungs was significantly increased in mice treated with 1000mg/kg UR. MDA
levels in lungs were significantly decreased in mice treated with 100mg/kg & 500 mg/kg UR but was
significantly increased in mice treated with 1000mg/kg UR. In kidneys, DTD activity was significantly increased
in mice treated with 1000mg/kg UR. In stomach, CAT activity was significantly increased in mice treated with
1000mg/kg UR. The results showed that Cosmos caudatus supplementation in mice could protect extrahepatic
organs from xenobiotic and oxidative injury. This indicates that consumption of ‘Ulam Raja’ might be a useful
chemoprotective measure.
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Keywords
Cosmos caudatus, mice, extrahepatic organs, detoxifying enzymes, antioxidant
Citation
Abdullah Azman, Dhaliwal Kezreen Kaur, Roslan Nur Nabillah Fahana, Lee Chai Hui, Kalaiselvam Murali, Miszard Harizz Radman. Saad Qodriyah Haji Mohd, Yusof Kamisah, Jaarin Kamsiah. The Effects of Cosmos caudatus (Ulam Raja) on Detoxifying Enzymes in Extrahepatic Organs in Mice. Journal of Applied Pharmaceutical Science. 2015 Jan; 5(1): 82-88.