Nephroprotective and Curative Assessment of an Aqueous Seed Extract of Parkia clappertoniana Keay in Gentamicin-induced Renal Damage in Sprague-dawley Rats.

dc.contributor.authorBoye, Alex
dc.contributor.authorKoffuor, George Asumeng
dc.contributor.authorAmpong, Joyce
dc.contributor.authorAnto, Enoch Odame
dc.contributor.authorOtoo, Lydia Francisca
dc.date.accessioned2015-10-08T07:30:59Z
dc.date.available2015-10-08T07:30:59Z
dc.date.issued2014-02
dc.description.abstractAim: To assess the nephroprotective and curative effects of an aqueous seed extract of Parkia clappertoniana on gentamicin-induced renal damage (GIRD) in Sprague-Dawley rats. Study Design: Experimental Place and Duration of Study: Department of Medical Laboratory, University of Cape Coast, Ghana between September, 2012 and May 2013. Methodology: In assessing nephro protective effect, rats were pretreated (10 days) with P. clappertoniana aqueous seed extract (1-2 g kg-1; p.o) prior to induction of renal damage by treatment with gentamicin (0.08g kg-1; p.o, for 8 days. Serum biochemical markers (Creatinine, Urea, Na+ and K+) and urine parameters (leukocyte, protein, specific gravity and pH) of renal damage were determined and compared with baseline values. In a curative study, GIRD in rats was treated with Normal Saline (2 ml kg-1; p.o), Losartan (0.05 g kg-1; p.o), or extract (1-2 g kg-1; p.o) for 14 days and serum and urine parameters determined for all treatments. Histopathology and changes in kidney weights for normal and treated rats in both studies were assessed. The extract was screened for DPPH radical scavenging activity. Results: The extract significantly (P ≤ .001) reduced elevated serum creatinine and urea secondary to GIRD (P ≤ .05) and significantly (P ≤ .05) reduced elevated serum Na+ but had no effect on K+. Elevated urine proteins and leucocytes secondary to GIRD was significantly (P ≤ .05) reduced; but had no significant effect on urine pH and specific gravity. Elevated kidney weights associated with GIRD was significantly (P≤ .01) reduced. Histopathological assessment revealed healing effect by extract to GIRD. Effects of the extract were similar to Losartan. Pretreatment with extract however had no significant effect on GIRD as serum and urine parameters, as well as kidney weights were significantly (P≤ .01) elevated on induction of renal damage. Conclusion: The aqueous seed extract of Parkia clappertoniana has curative but no nephroprotective effect on gentamicin-induced renal damage in Sprague-Dawley rats.en_US
dc.identifier.citationBoye Alex, Koffuor George Asumeng, Ampong Joyce, Anto Enoch Odame, Otoo Lydia Francisca. Nephroprotective and Curative Assessment of an Aqueous Seed Extract of Parkia clappertoniana Keay in Gentamicin-induced Renal Damage in Sprague-dawley Rats. European Journal of Medicinal Plants. 2014 Feb; 4(2): 234-248.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/164088
dc.language.isoenen_US
dc.source.urihttps://sciencedomain.org/abstract/2751en_US
dc.subjectPlasma creatinineen_US
dc.subjectPlasma ureaen_US
dc.subjectAzotemiaen_US
dc.subjectProteinuriaen_US
dc.subjectHypersthenuria reactiveen_US
dc.subjectoxygen speciesen_US
dc.subjectSaponinsen_US
dc.subjectPolyuronidesen_US
dc.titleNephroprotective and Curative Assessment of an Aqueous Seed Extract of Parkia clappertoniana Keay in Gentamicin-induced Renal Damage in Sprague-dawley Rats.en_US
dc.typeArticleen_US
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