Isolation and identification of poly beta hydroxybutyric acid accumulating bacteria of Staphylococcal sp. and characterization of biodegradable polyester.

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dc.contributor.advisorSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.contributor.authorRoy, Bappadityaen_US
dc.contributor.authorBanerjee, Rajaten_US
dc.contributor.authorChatterjee, Sumanaen_US
dc.date.accessioned2009-05-28T13:39:07Z
dc.date.available2009-05-28T13:39:07Z
dc.date.issued2009-04-23en_US
dc.description.abstractStaphylococcus sp. strain BP/SU1, capable of degrading the biopolymer and utilize it as a source of carbon and energy, was isolated from activated sludge using METABOLIX (MBX D411G). It was found that this strain was capable of accumulating poly(3-hydroxybutyric acid) P(3-HB), as granule poly (3-hydroxybutyric acid), p(3-HB), inclusion bodies when grown under suitable nutrient conditions. These strains could sustain cell growth up to a dry mass of 9.24 g/l with a doubling time of 8 to 10 hr and could accumulate P(3-HB) as granular inclusion bodies to a cell dry weight of more than 12%. P(3-HB) accumulated by this organism was isolated and characterized through NMR, FT-IR spectroscopy, UV Spectroscopy, Mass spectroscopy and Differential Scanning Calorimetry. P(3-HB) granules so isolated showed physical and chemical properties that should be possessed by a superior quality thermoplastic biopolymer.en_US
dc.description.affiliationDr B C Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019.en_US
dc.identifier.citationRoy B, Banerjee R, Chatterjee S. Isolation and identification of poly beta hydroxybutyric acid accumulating bacteria of Staphylococcal sp. and characterization of biodegradable polyester. Indian Journal of Experimental Biology. 2009 Apr; 47(4): 250-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/59689
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/3855/1/IJEB%2047%284%29%20250-256.pdfen_US
dc.subject.meshHydroxybutyrates --chemistry
dc.subject.meshHydroxybutyrates --metabolism
dc.subject.meshMagnetic Resonance Spectroscopy
dc.subject.meshPolyesters --chemistry
dc.subject.meshPolyesters --metabolism
dc.subject.meshSewage --microbiology
dc.subject.meshSpectrophotometry, Ultraviolet
dc.subject.meshStaphylococcus --cytology
dc.subject.meshStaphylococcus --growth & development
dc.subject.meshStaphylococcus --isolation & purification
dc.subject.meshStaphylococcus --metabolism
dc.titleIsolation and identification of poly beta hydroxybutyric acid accumulating bacteria of Staphylococcal sp. and characterization of biodegradable polyester.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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