Inhibition of azoxymethane-induced DNA adduct formation by Aloe arborescens var. natalensis.

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dc.contributor.authorShimpo, Kanen_US
dc.contributor.authorChihara, Takeshien_US
dc.contributor.authorBeppu, Hidehikoen_US
dc.contributor.authorIda, Chikakoen_US
dc.contributor.authorKaneko, Takaakien_US
dc.contributor.authorHoshino, Motoyukien_US
dc.contributor.authorKuzuya, Hiroshien_US
dc.date.accessioned2009-05-27T17:52:14Z
dc.date.available2009-05-27T17:52:14Z
dc.date.issued2003-07-26en_US
dc.descriptionAsian Pacific Journal of Cancer Prevention.en_US
dc.description.abstractTo clarify the possible mechanisms of inhibition of azoxymethane (AOM)-induced aberrant crypt foci (ACF) in the rat colorectum by freeze-dried whole leaves of Aloe arborescens var. natalensis (Kidachi aloe) (hereinafter referred to as ALOE) and commercial crude aloin (Sigma A-0451; from Curacao aloe) (hereinafter ALOIN), we studied the effects of ALOE and ALOIN on the formation of AOM-induced DNA adducts (O6-methylguanine; O6-MeG) in rats. Male F344 rats (4 weeks old) were fed a basal diet, or experimental diets containing 5%ALOE or 0.25%ALOIN for 5 weeks. All rats were injected s.c. twice with 15 mg/kg AOM, once at the end of week 1, and once at the end of week 2. The animals were sacrificed 6 hours after the second injection to analyze DNA adducts (O6-MeG) in the colorectum. Dietary administration of ALOE significantly inhibited the O6-MeG levels (50% reduction) compared with controls, whereas the O6-MeG levels in the ALOIN-fed rats showed a tendency to decrease (by 30%), although not significantly. In this study, we also measured the enzyme activity and mRNA level of cytochrome (CYP) 2E1, known to be responsible for the activation of AOM, in rat liver. ALOE-fed rats showed significantly reduced CYP2E1 enzymatic activity (27% reduction) compared with controls. On the other hand, the activity in ALOIN-fed rats tended to decrease by 11%, although not significantly. The CYP2E1 mRNA levels in ALOE- and ALOIN-fed rats were slightly reduced (9.7% and 5.2%, respectively). These results may explain, at least in part, the previously observed inhibitory effects of ALOE and ALOIN, especially ALOE on AOM-induced ACF formation in the rat colorectum.en_US
dc.description.affiliationFujita Memorial Institute of Pharmacognosy, Fujita Health University, Hisai, Mie 514-1296, Japan. shimpo@fujita-hu.ac.jpen_US
dc.identifier.citationShimpo K, Chihara T, Beppu H, Ida C, Kaneko T, Hoshino M, Kuzuya H. Inhibition of azoxymethane-induced DNA adduct formation by Aloe arborescens var. natalensis. Asian Pacific Journal of Cancer Prevention. 2003 Jul-Sep; 4(3): 247-51en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/38059
dc.language.isoengen_US
dc.source.urihttps://www.apocp.orgen_US
dc.subject.meshAloeen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnticarcinogenic Agents --pharmacologyen_US
dc.subject.meshAzoxymethane --antagonists & inhibitorsen_US
dc.subject.meshCytochrome P-450 CYP2E1 --geneticsen_US
dc.subject.meshDNA Adducts --drug effectsen_US
dc.subject.meshEmodin --analogs & derivativesen_US
dc.subject.meshMaleen_US
dc.subject.meshRNA, Messenger --metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred F344en_US
dc.titleInhibition of azoxymethane-induced DNA adduct formation by Aloe arborescens var. natalensis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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