Impact of HCV Genome Structure on Responder to Pegylated Interferon Therapy in Egyptian Patients

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dc.contributor.authorSanaa, Osama Abdallahen_US
dc.contributor.authorMohamed, Ali El-Dessoukyen_US
dc.contributor.authorMoataz, Hassan Hassaneinen_US
dc.contributor.authorHanan, Omaren_US
dc.contributor.authorKhaled, Mohamed Farid El Sehrawyen_US
dc.date.accessioned2020-09-24T07:28:32Z
dc.date.available2020-09-24T07:28:32Z
dc.date.issued2019-03
dc.description.abstractThis study aimed to investigate relationship of the HCV genome structure and treatment with Pegylated Interferonα/Ribavirin (peg-IFNα/RBV) Egyptian patient. Mutations in two sites of HCV genome; the internal ribosome entry site(IRES) and the interferon sensitivity determining region (ISDR) of HCV genotype 4a were studied in details including DNAsequences and mutations detection in response to treatment. Ninety patients, responders and non-responders, to treatmentwith peg-IFN α /RBV were included in this study. IRES and ISDR regions were amplified by RT-PCR using specific designedprimers, and amplified regions were sequenced. The data obtained were aligned with published sequences in GenBank usingBLAST program. Results of this study have revealed that there are different mutations in the studied sequences in both ISDRand IRES regions. The predicted amino acids sequences in the ISDR region showed significant differences ranging from oneup to more than eight mutations in the HCV Genome sequences. Although there was a significant difference betweensequences of HCV RNA isolated from responders and non-responders, these data were not able to give an absolute answerwhether response to interferon therapy is directly/relates to the structure of the HCV genomeen_US
dc.identifier.affiliationsChemistry Department, Faculty of Science, Cairo University, Egypten_US
dc.identifier.affiliationsChemistry Department, Faculty of Science, Cairo University, Egypten_US
dc.identifier.affiliationsHepatology and Gastroenterology, Department, Theodor Bilharz Research Institute, Giza Egypten_US
dc.identifier.affiliationsBiochemistry and Molecular Biology Department, Theodor Bilharz Research Institute, Giza Egypten_US
dc.identifier.affiliationsChemistry Department, Faculty of Science, Cairo University, Egypt,en_US
dc.identifier.citationSanaa Osama Abdallah, Mohamed Ali El-Dessouky, Moataz Hassan Hassanein, Hanan Omar, Khaled Mohamed Farid El Sehrawy. Impact of HCV Genome Structure on Responder to Pegylated Interferon Therapy in Egyptian Patients . International Journal of Medical Research Professionals. 2019 Sep; 8(3): 61-68en_US
dc.identifier.issn2277-3657
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/203649
dc.languageenen_US
dc.publisherInternational Journal of Pharmaceutical Research and Allied Sciencesen_US
dc.relation.issuenumber3en_US
dc.relation.volume8en_US
dc.source.urihttps://ijpras.com/storage/models/article/64Ugx3SgDX7FkQfEkDhgHOSbRpMAhVbNxSsRUxz4znU0fs4HsRIb9fYfSbuJ/impact-of-hcv-genome-structure-on-responder-to-pegylated-interferon-therapy-in-egyptian-patients.pdfen_US
dc.subjectHepatitis C virusen_US
dc.subjectPegylated Interferon-αen_US
dc.subjectIRESen_US
dc.subjectISDR.en_US
dc.titleImpact of HCV Genome Structure on Responder to Pegylated Interferon Therapy in Egyptian Patientsen_US
dc.typeJournal Articleen_US
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