High frequency axillary bud multiplication and ex vitro rooting of Wedelia chinensis (Osbeck) Merr.--a medicinal plant.

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dc.contributor.authorMartin, K Pen_US
dc.contributor.authorBeena, M Ren_US
dc.contributor.authorJoseph, Dominicen_US
dc.date.accessioned2009-05-28T10:57:25Z
dc.date.available2009-05-28T10:57:25Z
dc.date.issued2003-03-23en_US
dc.description.abstractAn efficient protocol was achieved for rapid propagation of Wedelia chinensis (Osbeck) Merr. through axillary bud proliferation and ex vitro rooting. Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 8.87 microM) and indole-3-butyric acid (IBA; 2.46 microM) was optimal for axillary bud proliferation, which developed a mean of 8.3 shoots/node. Excision and culture of node segments from in vitro shoots on medium supplemented with the same concentration of growth regulators developed more than 30 shoots within 40 days. Excision and culture of nodes in succession enhanced the number of shoots. Shoot multiplication did not exhibit decrease in the number of shoots even at 10th subculture. Nevertheless, the shoots exhibited a tendency towards stunted nature. But reduction of BA to 4.44 or 2.22 microM resumed normal growth of shoots. Half strength MS medium fortified with IBA (2.46 microM) induced the highest number of roots. All in vitro rooted shoots survived in field. Dipping of the basal end of shoots collected from multiplication medium in IBA (2.46 microM) solution for 7 days induced roots and its transfer to small pots facilitated the survival of all rooted shoots (100%). Rooting ex vitro by direct transfer of shoots from multiplication medium exhibited 89.2 per cent survival. Use of commercial sugar and tap water and also the omission of in vitro rooting reduce the propagation cost 50-70 per cent. The protocol enables to harvest more than 50,000 plantlets within 150 days starting from a single node explant.en_US
dc.description.affiliationDepartment of Biotechnology, University of Calicut, Calicut 673 635, India. marttin@sify.comen_US
dc.identifier.citationMartin KP, Beena MR, Joseph D. High frequency axillary bud multiplication and ex vitro rooting of Wedelia chinensis (Osbeck) Merr.--a medicinal plant. Indian Journal of Experimental Biology. 2003 Mar; 41(3): 262-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56036
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshPlant Roots --growth & developmenten_US
dc.subject.meshPlants, Medicinal --growth & developmenten_US
dc.subject.meshWedelia --growth & developmenten_US
dc.titleHigh frequency axillary bud multiplication and ex vitro rooting of Wedelia chinensis (Osbeck) Merr.--a medicinal plant.en_US
dc.typeJournal Articleen_US
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