Antioxidant and hepatoprotective activity of Flemingia tuberosa Dalzell in CCl4-induced hepatotoxicity in rats
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Date
2020-06
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Flemingia tuberosa Dalzell is an endemic species of the Western Ghats. It is an ethnomedicinal plant used to curedysentery and vaginal discharge. However, in this study, the hepatoprotective potency of the plant has been evaluatedas it contains flavonoids like kaempferol. The present study was conducted to investigate the hepatoprotective activityof the ethanolic extract of aerial parts of F. tuberosa against Carbon tetrachloride (CCl4)-induced toxicity in rats.The extract was standardized using a validated HPTLC method for kaempferol content. The antioxidant activityof the ethanolic extract of aerial parts of F. tuberosa was assessed by 1,1-diphenyl-2-picrylhydrazyl method. Thehepatoprotective activity was assessed against CCl4-induced liver intoxication, wherein the CCl4-induced albino Wistarrats were treated with three different doses of the standardized extract for 3 days. The results were compared with theestablished positive control, Silymarin. The protective effect on the liver was also monitored histopathologically usinglight and electron microscope and biochemically through observing the levels of biochemical enzymes. The extractshowed potent hepatoprotective activity. This study confirms the protective effect of the ethanolic extract of aerialparts of F. tuberosa against CCl4-induced hepatotoxicity
Description
Keywords
Flemingia, endemic, chromatography, antioxidant, hepatoprotective, histopathology
Citation
Mascarenhas Reena William, Shailajan Sunita, Menon Sasikumar. Antioxidant and hepatoprotective activity of Flemingia tuberosa Dalzell in CCl4-induced hepatotoxicity in rats. Journal of Applied Pharmaceutical Science. 2020 Jun; 2020 Jun: 032-038