Growth Inhibitory Activity of Garcinia Hanburyi extracts on Cholangiocarcinoma Cell Lines.

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dc.contributor.authorWongwarut Boonyanugomolen_US
dc.contributor.authorChariya Hahnvajanawongen_US
dc.contributor.authorVichai Reutrakulen_US
dc.contributor.authorNatthinee Anantachokeen_US
dc.date.accessioned2011-02-24T04:34:31Z
dc.date.available2011-02-24T04:34:31Z
dc.date.created2010-05-29en_US
dc.date.issued2010-05-29en_US
dc.description.abstractBackground: Cholangiocarcinoma (CCA) is the highest incident of primary liver cancer in the northeastern  Thailand. All of the treatment approaches remain poor. One attractive strategy is the discovery of new drugs from medicinal plants which have a potential growth inhibitory activity on cholangiocarcinoma cells.Objective: To examine the growth inhibitory activity of crude extracts and fractions from barks and fruits of Garcinia hanburyi on human cholangiocarcinoma cell lines.Method: Growth inhibitory activity was assessed by sulforhodamine B assay. The mean IC50 values of three independent experiments were reported.Results: The growth inhibitory activity of four extracts from barks and fruits of G. hanburyi on five human CCA cell lines was performed. It was found that the ethyl acetate extracts from barks (VR12874) and fruits (VR11626) of G. hanburyi exhibited strong growth inhibitory activity against five CCA cell lines with IC50 values range from 1.84±0.10 to 2.49±0.03 mg/ml and 1.69±0.04 to 4.41±0.10 mg/ml, respectively whereas the methanol extracts from barks (VR12875) and fruits (VR11627) showed no growth inhibitory activity on CCA cell lines tested. Subsequent to these results eight fractions from VR12874 were examined for their growth inhibitory activities against 5 human CCA cell lines. It was found that five fractions including  VR12877, VR12878, VR12881, VR12882 and VR12883 showed strong growth inhibitory activity whereas VR12876 and VR12879 exhibited low growth inhibitory activity and VR12880 showed no effect.Discussion and conclusion: It could be concluded that the ethyl acetate extracts from barks and fruits of G. hanburyi showed strong growth inhibitory effect against 5 human CCA cell lines whereas the methanol extracts had no effect. These results indicated that the ethyl acetate extracts might contain active constituents. Variation in the growth inhibitory activities among fractions might  depend on the type or concentration of active constituents containing in each fractions.Key words: Garcinia hanburyi, growth inhibitory activity, cholangiocarcinoma cell lines en_US
dc.identifier.citationSrinagarind Medical Journal (SMJ); Vo. 22 No. 3 July - September 2007; 278-282en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/133996
dc.language.isoen_USen_US
dc.publisherFaculty of Medicine, Khon Kaen Universityen_US
dc.rightsAcademic Affairs, Faculty of Medicine, Khon Kaen University, KhonKaen, Thailanden_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/SMJ/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/SMJ/article/view/1894en_US
dc.titleGrowth Inhibitory Activity of Garcinia Hanburyi extracts on Cholangiocarcinoma Cell Lines.en_US
dc.typeOriginal Articlesen_US
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