Validation of PCR based detection system for aflatoxin producing molds.

Simple item page
dc.contributor.authorMangal, Manisha
dc.contributor.authorKhan, Farha
dc.contributor.authorBansal, Sangita
dc.contributor.authorOberoi, HS
dc.date.accessioned2016-11-24T05:00:11Z
dc.date.available2016-11-24T05:00:11Z
dc.date.issued2016-07
dc.description.abstractAflatoxins are polyketide secondary metabolites that are produced by certain fungal species in the Aspergillus section Flavi, particularly Aspergillus flavus and Aspergillus parasiticus which contaminate human food as well as animal feed. These are among the most carcinogenic substances known. Due to the toxic and carcinogenic properties of aflatoxins, there is a need to develop reliable methods to detect the presence of aflatoxigenic Aspergilli in contaminated food and feed. Not all Aspergillus strains are able to produce aflatoxins. It requires a detection methodology which can specifically distinguish between the aflatoxin producing and non-producing strains of Aspergillus. Present communication reports validation of a PCR based detection system based on three genes viz., nor-1, apa-2 and omt-1 involved in aflatoxin biosynthesis, that can specifically distinguish the two aflatoxin producing species viz. Aspergillus flavus and Aspergillus parasiticus from non-producers i.e., A. niger, A. fumigates and A. oryzae.en_US
dc.identifier.citationMangal, Manisha Khan, Farha Bansal, Sangita Oberoi, HS. Validation of PCR based detection system for aflatoxin producing molds. Indian Journal of Experimental Biology. 2016 July; 54(7): 472-476.en_US
dc.identifier.issn0975-1009
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/178777
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/34749en_US
dc.subjectAspergillus flavusen_US
dc.subjectAspergillus parasiticusen_US
dc.subjectContaminationen_US
dc.subjectFood safetyen_US
dc.subjectFungien_US
dc.subjectMycotoxinsen_US
dc.titleValidation of PCR based detection system for aflatoxin producing molds.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijeb2016v54n7p472.pdf
Size:
145.75 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Indian Journal of Experimental Biology