Alterations in brain tumor DNA detected by a fingerprinting probe.

Joshi, A R; Sinha, S; Dil-Afroz,; Sulaiman, I M; Banerji, A K; Hasnain, S E

Alterations in brain tumor DNA detected by a fingerprinting probe.

Date

1996-12-01

Authors

Abstract

We used a novel DNA fingerprinting probe O-chi-1 (ref. 1) to detect differences in the hybridization pattern of brain tumor DNA and paired normal tissue of a given individual. Representatives of meningiomas (two), glioblastoma multeforme (three) and astrocytoma (one) were studied. Alterations, which included amplification as well as the loss of a normal band in tumor DNA, were observed in four of the six tumours. While the increased intensity of a band can be taken to imply increased copy number, the disappearance of bands could either be due to loss of DNA sequence or rearrangement resulting in different sized bands.

Citation

Joshi AR, Sinha S, Dil-Afroz , Sulaiman IM, Banerji AK, Hasnain SE. Alterations in brain tumor DNA detected by a fingerprinting probe. Indian Journal of Biochemistry & Biophysics. 1996 Dec; 33(6): 455-7