Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain.
dc.contributor.author | Raj, S K | en_US |
dc.contributor.author | Leiser, R M | en_US |
dc.contributor.author | Plobner, L | en_US |
dc.date.accessioned | 2009-05-28T11:31:23Z | |
dc.date.available | 2009-05-28T11:31:23Z | |
dc.date.issued | 1994-05-01 | en_US |
dc.description.abstract | RNA isolation from purified cucumber mosaic virus, CMV-U strain and cDNA synthesis was carried out. The coat protein gene (RNA4) region was amplified selectively by polymerase chain reaction (PCR) with CMV RNA4-specific primers. Double-stranded cDNA was cloned in PRT103 vector at Xho1/Kpn1 site and about 1kb insert obtained. The insert was partly sequenced which showed 50% sequence homology with CMV-Q, C and WL strains. | en_US |
dc.description.affiliation | Institute of Genitics & Crop Research, Gatersleben, Germany. | en_US |
dc.identifier.citation | Raj SK, Leiser RM, Plobner L. Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain. Indian Journal of Experimental Biology. 1994 May; 32(5): 345-7 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/56832 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.asp | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Capsid --genetics | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Cucumovirus --genetics | en_US |
dc.subject.mesh | Genes, Viral | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.title | Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain. | en_US |
dc.type | Journal Article | en_US |
dc.type | Research Support, Non-U.S. Gov't | en_US |
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