Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain.

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dc.contributor.authorRaj, S Ken_US
dc.contributor.authorLeiser, R Men_US
dc.contributor.authorPlobner, Len_US
dc.date.accessioned2009-05-28T11:31:23Z
dc.date.available2009-05-28T11:31:23Z
dc.date.issued1994-05-01en_US
dc.description.abstractRNA isolation from purified cucumber mosaic virus, CMV-U strain and cDNA synthesis was carried out. The coat protein gene (RNA4) region was amplified selectively by polymerase chain reaction (PCR) with CMV RNA4-specific primers. Double-stranded cDNA was cloned in PRT103 vector at Xho1/Kpn1 site and about 1kb insert obtained. The insert was partly sequenced which showed 50% sequence homology with CMV-Q, C and WL strains.en_US
dc.description.affiliationInstitute of Genitics & Crop Research, Gatersleben, Germany.en_US
dc.identifier.citationRaj SK, Leiser RM, Plobner L. Molecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain. Indian Journal of Experimental Biology. 1994 May; 32(5): 345-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/56832
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCapsid --geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshCucumovirus --geneticsen_US
dc.subject.meshGenes, Viralen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.titleMolecular cloning of coat protein gene of cucumber mosaic virus, CMV-U strain.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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