Effect of KATP channel openers on myogenic and neurogenic responses in goat urinary bladder.

Simple item page
dc.contributor.authorVijayakumar, Cen_US
dc.contributor.authorKathirvel, Ken_US
dc.contributor.authorSardar, K Ken_US
dc.contributor.authorParija, S Cen_US
dc.date.accessioned2009-05-28T10:41:13Z
dc.date.available2009-05-28T10:41:13Z
dc.date.issued2007-02-23en_US
dc.description.abstractIsolated goat detrusor muscle exhibited spontaneous contractility with an irregular amplitude and frequency. The spontaneity of detrusor muscle exhibited a mean amplitude as 11.99 +/- 0.83 mm and frequency as 1.37 +/- 0.16/min. KATP-channel openers namely, cromakalim or pinacidil (10(-7) - 10(-4) M) added cumulatively, elicited a concentration-related inhibition of both amplitude and rate of spontaneous contractions. The mean IC50 values for both amplitude and frequency for cromakalim were 3.3 x 10(-6) M and 2.9 x 10(-6) M, respectively; and for pinacidil were 2.0 x 10(-5) M and 1.5 x 10(-5) M, respectively. Glibenclamide, a KATP-channel blocker inhibited the cromakalim-induced concentration-related relaxation of spontaneous contractions with a significant increase in its mean IC50. ACh-induced concentration-related contractile response was inhibited in the presence of either cromakalim (10(-4) M) or pinacidil (10(-4) M). The mean EC50 value of ACh, in the presence of cromakalim (2.5 x 10(-3) M) was significantly increased as compared to the control (1.2 x 10(-6) M). In the presence of glibenclamide (10(-5) M) the inhibitory effect of cromakalim was significantly reduced with consequent decrease in the EC50 value (1.9 x 10(-5) M). Application of EFS (30 V and 5 ms) on goat urinary bladder strips at 1, 2, 5, 10, 20 and 30 Hz elicited frequency-related contractile responses. Both cromakalim and pinacidil caused a rightward shift in the frequency-related contractile response curve with significant increase in the mean EF25 and EF50 values, respectively. In the presence of glibenclamide (10(-4) M), the frequency-related inhibitory response curve was shifted to left with significant (P < 0.001) increase in the mean EF25, EF50 and EF75. The present results suggest that in the goat detrusor muscle, agonist and EFS-induced contractile responses were more potently inhibited by cromakalim than pinacidil with activation of glibenclamide sensitive KATP channels.en_US
dc.description.affiliationDepartment of Pharmacology and Toxicology, Faculty of Veterinary Sciences and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar 751 003, India.en_US
dc.identifier.citationVijayakumar C, Kathirvel K, Sardar KK, Parija SC. Effect of KATP channel openers on myogenic and neurogenic responses in goat urinary bladder. Indian Journal of Experimental Biology. 2007 Feb; 45(2): 185-93en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/55654
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/5230/1/IJEB%2045%282%29%20185-193.pdfen_US
dc.subject.meshAcetylcholine --pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAtropine --pharmacologyen_US
dc.subject.meshCholinergic Agents --pharmacologyen_US
dc.subject.meshCromakalim --pharmacologyen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshFemaleen_US
dc.subject.meshGlyburide --pharmacologyen_US
dc.subject.meshGoats --physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMuscarinic Antagonists --pharmacologyen_US
dc.subject.meshMuscle Contraction --drug effectsen_US
dc.subject.meshMuscle, Smooth --physiologyen_US
dc.subject.meshPinacidil --pharmacologyen_US
dc.subject.meshPotassium Channel Blockers --pharmacologyen_US
dc.subject.meshPotassium Channels --agonistsen_US
dc.subject.meshUrinary Bladder --drug effectsen_US
dc.titleEffect of KATP channel openers on myogenic and neurogenic responses in goat urinary bladder.en_US
dc.typeIn Vitroen_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Experimental Biology