Immunological identification of two female-specific proteins from the plasma of Indian freshwater murrel, Channa punctatus (Bloch).

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dc.contributor.authorSehgal, Neetaen_US
dc.contributor.authorGoswami, S Ven_US
dc.date.accessioned2009-05-28T14:12:53Z
dc.date.available2009-05-28T14:12:53Z
dc.date.issued2002-03-15en_US
dc.description.abstractExistence of a non-phosphorylated female-specific protein (FS II), in addition to phosphorylated vitellogenin (FS I), in the plasma of murrel by exogenous administration of estradiol-17beta is reported. Polyspecific rabbit antibodies were raised against estrogen-inducible murrel plasma proteins. This antiserum was absorbed with normal male serum in order to obtain female-specific antiserum (FSAS). Radial immunodiffusion studies suggested that both the proteins (FS I and FS II) were present in the plasma of E2-treated and normal vitellogenic females and in the ovarian homogenate from gravid females, but absent in normal male plasma. Autoradiographic experiments demonstrated that phosphorus moiety was attached with FS I only. Further, immunoelectrophoretic analysis and peptide maps supported the observation that FS I and FS II were discrete, unrelated female-specific proteins.en_US
dc.description.affiliationDepartment of Zoology, University of Delhi, Delhi 110 007, India.en_US
dc.identifier.citationSehgal N, Goswami SV. Immunological identification of two female-specific proteins from the plasma of Indian freshwater murrel, Channa punctatus (Bloch). Indian Journal of Experimental Biology. 2002 Mar; 40(3): 288-95en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60418
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibody Specificityen_US
dc.subject.meshBlood Proteins --immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshImmunochemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshPerciformes --blooden_US
dc.subject.meshSex Characteristicsen_US
dc.subject.meshVitellogenins --blooden_US
dc.titleImmunological identification of two female-specific proteins from the plasma of Indian freshwater murrel, Channa punctatus (Bloch).en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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