Light and transmission electron microscopic studies of the third stage larvae of anisakis simplex.
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Date
2012-01
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Abstract
Objective: The purpose of this study was to demonstrate the structures of the third stage larva of Anisakis simplex in marine
fish by using light microscope (LM) and transmission and transmission electron microscope (TEM).
Methods: L3 Anisakis simplex were processed for conventional light and electron microscopic studies.
Results: The body wall of the L3 A. simplex is composed of three layers, the cuticle, hypodermis and somatic muscular layer
from the superficial to deep surface of the worm, respectively. The cuticle is thick, functions as a protective barrier and barring
the antigenic molecules. Furthermore, TEM reveals that the cuticle is subdivided into cortical, middle and basal layers from
outer to inner part of the cuticle. This layer is highly filamentous and the filaments arrange randomly in several directions.
The hypodermis is a thin layer which functions as the cuticular productive layer. The lateral hypodermal cords are bilobed.
The somatic muscular layer is composed of a single row of muscle cells, which lie along the long axis of the body. The
gastrointestinal tract of the worm is a straight tube, lined with stratified epithelium and surrounded by the basal lamina. The
intestinal epithelial cells contain various organelles, which its luminal surface presents numerous microvilli for absorption of
nutrient molecules.
Conclusion: The cross section of LM and TEM can be used to distinguish the nematode species, as the LM reveals the lateral
cords of L3 A. simplex are bilobed structures which are different from other species.
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Keywords
L3 Anisakis implex, LM, TEM
Citation
Roongruangchai Jantima, Tamepattanapongsa Apichat, Roongruangchai Kosol. Light and transmission electron microscopic studies of the third stage larvae of anisakis simplex. Siriraj Medical Journal, 2012 Jan; 64(suppl): 72-77.