Antibacterial, Molecular Docking, DNA Binding and Photocleavage Studies on Novel Heterocyclic Pyrazoles.
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Date
2011-07
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Abstract
The antibacterial activity, in-silico DNA molecular docking, DNA binding and photo
cleavage studies of newly synthesized pyrazole is described. Antibacterial potential of
these compounds screened against a wide range of Gram-positive and Gram-negative
bacteria showed significant zone of inhibition and MIC with standard drug ciproflaxin is
investigated. Among all the orientation of binding, fourth orientation showed significant
binding and revealed that the binding and docking energy of 4a was -8.62 and -8.66
and inhibition constant 7.46 X e-6. The absorption spectra showed the dynamic
interaction with CT DNA and as proficient DNA intercalator (Kb = 4.5×104 M-1). The
viscosity measurements and thermal denaturation affords the positive results towards
DNA intercalation in both the studies. The light induced DNA damage was pragmatic in
the absence of various ‘‘inhibitors’’ shows in photo cleavage activities at 360 nm.
Description
Keywords
CT-DNA, intercalator, in-silico, molecular docking, photocleavage, pyrazole
Citation
Sharath N, Naik Halehatty S Bhojya, Kumar B Vinay, Hoskeri Joy. Antibacterial, Molecular Docking, DNA Binding and Photocleavage Studies on Novel Heterocyclic Pyrazoles. British Journal of Pharmaceutical Research. 2011 Jul-Sep; 1(3): 46-65.