Stereological study of rat spleen following acute ethanol treatment.

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dc.contributor.authorBudec, Men_US
dc.contributor.authorMilićević, Zen_US
dc.contributor.authorKoko, Ven_US
dc.date.accessioned2009-05-28T11:45:04Z
dc.date.available2009-05-28T11:45:04Z
dc.date.issued2000-05-29en_US
dc.description.abstractTo investigate the acute effect of ethanol (4 g/kg, i.p.) on spleen adult female Wistar rats were treated intraperitoneally with: a) ethanol (4 g/kg body wt), b) naltrexone (5 mg/kg body wt) followed 45 minutes later by ethanol (4 g/kg body wt) and c) naltrexone (5 mg/kg body wt) alone. Untreated and saline-treated rats were used as controls. Twenty hours after the ethanol treatment the animals were sacrificed and the spleens were removed. A piece of tissue from the central part of each organ was fixed in Bouin's solution. Paraffin sections were stained with hematoxylin-eosin and analysed using stereological measurements. The volume densities of the following tissue compartments: red pulp, white pulp (divided in follicles, periarterioral lymphatic sheath and marginal zone) and the connective tissue were determined. Stereological analysis also included parameters of follicles: the areal numerical density (the number of follicles per 1 mm2 of tissue section), the numerical density (the number of follicles per mm3 of tissue) and the mean follicle diameter. The immunoarchitecture of the spleen was preserved following acute ethanol treatment. Unlike other parameters that were unaffected, ethanol evoked a decrease in both volume density of follicle and the mean follicle diameter. Naltrexone pretreatment had no influence on ethanol-induced changes. The data obtained indicate that a single dose of ethanol has a profound effect on rat spleen affecting the follicles, but the mechanism of its action remains to be elucidated.en_US
dc.description.affiliationInstitute for Medical Research, Medical Faculty, Beograd, Yugoslavia. mirelab@imi.bg.ac.yuen_US
dc.identifier.citationBudec M, Milićević Z, Koko V. Stereological study of rat spleen following acute ethanol treatment. Indian Journal of Experimental Biology. 2000 May; 38(5): 462-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/57149
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshEthanol --toxicityen_US
dc.subject.meshFemaleen_US
dc.subject.meshNaltrexone --pharmacologyen_US
dc.subject.meshNarcotic Antagonists --pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.subject.meshReceptors, Opioid --drug effectsen_US
dc.subject.meshSpleen --drug effectsen_US
dc.titleStereological study of rat spleen following acute ethanol treatment.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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