Effect of Desiccation and Chilling Treatment on Somatic Embryo Development and Germination in Rough Lemon (Citrus jambhiri Lush).
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Date
2014-02
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Abstract
The goal of this study was to evaluate the effect of desiccation and chilling treatments on
somatic embryogenesis of rough lemon (Citrus jambhiri Lush.). Styles were cultured on
seven culture media (MS I-MS VII) containing Benzylaminopurine (BAP), Kinetin (KN) and
Malt Extract for cell proliferation and somatic embryo development. Cell proliferation was
maximum on MS IV media but maximum cultures showing somatic embryogenesis (52.08
%) was observed on MS VII media. Embryogenic callus proliferated on MS VII media was
subjected to desiccation and chilling treatment for 24, 48, 72 and 96 hours. Embryogenic
callus desiccated for 24 and 48 hours in sterile petriplates showed 58.33 and 56.94 %
somatic embryogenesis respectively as compared to undesiccated callus (51.98%).
Average number of cotyledonary embryos (6.80/culture) in embryogenic cultures from
desiccated callus (48 hrs) was more as compared to untreated callus (2.26/culture). There
was significantly less number of abnormal embryos (0.60-0.53/culture) in desiccated
callus for 48, 72 and 96 hours as compared to untreated callus (7.20/culture). Chilling
treatment also improves the average number of cotyledonary embryos and reduces the
abnormal development of embryos. Among all the treatments desiccation of embryogenic
callus for 48 hrs proved beneficial for improvement of somatic embryo development and
germination.
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Keywords
Citrus, embryogenic callus, desiccation, chilling, somatic embryogenesis
Citation
P V Krishna, K Madhusudhana Rao, V Swaruparani, D Srinivas Rao. Effect of Desiccation and Chilling Treatment on Somatic Embryo Development and Germination in Rough Lemon (Citrus jambhiri Lush). British Biotechnology Journal. 2014 Feb; 4(2): 136-148.