Cryopreservation of mouse embryos at -196 degrees C by vitrification.

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dc.contributor.authorAgrawal, K Pen_US
dc.contributor.authorPolge, Cen_US
dc.date.accessioned2009-05-28T14:11:05Z
dc.date.available2009-05-28T14:11:05Z
dc.date.issued1989-04-01en_US
dc.description.abstractEmbryos (8-16 cell) were obtained from random bred albino mice (6-8 weeks old) that were induced to superovulate by injections of 5 I.U. PMSG and 5 I.U. hCG given 48 hr apart. Embryos were exposed to intracellular cryoprotecting medium (glycerol 10%, 1-2 propanediol 20% in PBS) for 10 min and then transferred to extracellular vitrification medium (25% glycerol, 25% 1-2 propanediol in PBS). Vitrification medium containing embryos, and diluent (1 M sucrose) were loaded in a straw and immediately plunged into liquid N2. After thawing at 20 degrees C, the contents of the straw were mixed by shaking (1 step dilution) and emptied in a petri dish. After 3 washings in culture medium the embryos were kept in CO2 incubator for further development. In 3-step dilution procedure the dilution of cryoprotectants was done in 0.5 and 0.25 M sucrose before culture. Embryos in 3-step dilution of cryoprotectants exhibited high survival as compared to 1-step dilution (20.23% vs 6.55%).en_US
dc.identifier.citationAgrawal KP, Polge C. Cryopreservation of mouse embryos at -196 degrees C by vitrification. Indian Journal of Experimental Biology. 1989 Apr; 27(4): 383-4en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60379
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCryopreservation --methodsen_US
dc.subject.meshCryoprotective Agentsen_US
dc.subject.meshEmbryo, Mammalian --physiologyen_US
dc.subject.meshMiceen_US
dc.subject.meshTissue Preservation --methodsen_US
dc.titleCryopreservation of mouse embryos at -196 degrees C by vitrification.en_US
dc.typeJournal Articleen_US
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