Callus induction, Indirect Organogenesis and Plantlet Regeneration from different explants of Vernonia anthelmintica (L.) Willd
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Date
2020-05
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Present study was conducted to standardize callus development and indirect organogenesis from differentexplants of Vernonia anthelmintica (L.) Willd. Among, the various hormonal combinations tested: IndoleAcetic Acid (IAA) and BA and IAA and Kinetin combinations were found to be optimum for inducing callusingwithin a time span of 10 days. Best callus response was observed in 1.5 mg l−1 IAA and 1.5 mg l−1 BA,which produced white friable callus. Best indirect shoot organogenesis was observed in 4 mg l−1 BA and6 mg l−1 kinetin. Elongated shoots when transferred on to half strength Murashige and Skoog (MS) Mediumsupplemented with Indole-3-butyric acid (IBA), rooting was observed. MS medium fortified with 2 mg l−1 IBAshowed better rooting than all other concentrations tested. This concentration produced maximum number ofroots and maximum percentage of rooting. Plantlets developed by indirect organogenesis of V. anthelminticawere successfully acclimatized to field conditions
Description
Keywords
Vernonia anthelmintica, callus induction, plant growth regulators, somaclones, indirect organogenesis
Citation
Maya Rajan, Soororbhavan Shahena, Chandran Vinaya, Mathew Linu. Callus induction, Indirect Organogenesis and Plantlet Regeneration from different explants of Vernonia anthelmintica (L.) Willd. Journal of Applied Biology & Biotechnology. 2020 May; 2020 May: 17-22