Isolation, purification and partial characterization of a haemolytic protein from Bidder's organ of toad Bufo melanostictus (Schneider).

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dc.contributor.authorGomes, Aen_US
dc.contributor.authorAlam, M Ien_US
dc.contributor.authorAuddy, Ben_US
dc.contributor.authorDasgupta, S Cen_US
dc.date.accessioned2009-05-28T13:37:11Z
dc.date.available2009-05-28T13:37:11Z
dc.date.issued1994-02-01en_US
dc.description.abstractA haemolytic protein toxin (BO-HT) from Bidder's organ of toad, B. melanostictus, purified by DEAE-cellulose column chromatography was electrophoretically homogeneous and was glycoprotein in nature (PAS-positive). The molecular weight was estimated to be 14.4 kDa by SDS-polyacrylamide gel electrophoresis. The sensitivity of the haemolysin of different RBC ghost cell preparation was in the order: buffalo > goat > ox > guinea pig > mice > human > chick > rabbit > rat. The haemolytic activity was increased with the decrease in RBC concentration and was produced over a wide range of temperature. Maximum haemolytic effect was produced at 2 hr of incubation. The toxin showed maximum activity at 3 and minimum at 10 pH. Divalent cations (Ca2+, Zn2+, Cu2+, Mg2+) showed inhibitory effect on BO-HT induced haemolysis, whereas sucrose, EDTA, cholesterol, 2-mercaptoethanol and oxygen did not alter the haemolytic activity. Haemolytic activity was reduced by proteolytic enzymes (trypsin, protease) and was totally antagonized by the toad serum.en_US
dc.description.affiliationDepartment of Physiology, University of Calcutta, India.en_US
dc.identifier.citationGomes A, Alam MI, Auddy B, Dasgupta SC. Isolation, purification and partial characterization of a haemolytic protein from Bidder's organ of toad Bufo melanostictus (Schneider). Indian Journal of Experimental Biology. 1994 Feb; 32(2): 119-23en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/59647
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBufonidae --metabolismen_US
dc.subject.meshEndocrine Glands --chemistryen_US
dc.subject.meshHemolysin Proteins --isolation & purificationen_US
dc.subject.meshMaleen_US
dc.subject.meshProteins --isolation & purificationen_US
dc.titleIsolation, purification and partial characterization of a haemolytic protein from Bidder's organ of toad Bufo melanostictus (Schneider).en_US
dc.typeJournal Articleen_US
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