Neomycin robertson’s cooked meat medium (N.RCM) for the selective isolation of anaerobes.
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Date
2010-11
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Abstract
As Robertson’s cooked meat medium (RCM) promotes the simultaneous
multiplication of facultative anaerobes along with anaerobes, it may be interfered with
the isolation rate of anaerobes. In the present study RCM was made selective to
overcome this difficulty by the incorporation of Neomycin. The efficacy of modified
RCM was evaluated by growing stock cultures of various anaerobes and aerobes well as
by inoculating clinical specimen directly and comparing the results with routine RCM.
Among the 160 stock cultures of aerobes 8 of the 28 neomycin resistant aerobes grew in
N.RCM but none of the 132 neomycin sensitive aerobes. Out of the 150 clinical samples
tested in the study,77 anaerobes and 97 aerobes were isolated from routine RCM whereas
89 anaerobes and 8 Neomycin resistant aerobes from NRCM (i.e. NRCM inhibited
91.7% of aerobes and gave an increased yield of 13.5% anaerobes).These findings
suggest that NRCM can be employed to suppress the aerobes and at the same time to
enhance the isolation of anaerobes.
Description
Keywords
RCM, Anaerobes, N.RCM
Citation
Antony Beena, Ramanath K, Shivaprasad Aparna, Shenoy Poornima. Neomycin robertson’s cooked meat medium (N.RCM) for the selective isolation of anaerobes. International Journal of Applied Biology and Pharmaceutical Technology. 2010 Nov-Dec; 1(3): 1239-1243.