Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol.

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dc.contributor.authorSharma, Men_US
dc.contributor.authorModgil, Men_US
dc.contributor.authorSharma, D Ren_US
dc.date.accessioned2009-05-28T14:11:21Z
dc.date.available2009-05-28T14:11:21Z
dc.date.issued2000-12-20en_US
dc.description.abstractSuccessful in vitro propagation of clonal apple rootstock MM106 was achieved by culturing axillary buds on MS basal medium with BAP (1 mg/L), GA3 (0.5 mg/L) and IBA (0.1 mg/L). Use of liquid medium (LM) in initial cultures reduced phenol exudation to a greater extent and gave maximum sprouting percentage when transferred to solid MS medium. Phloroglucinol (PG) did not enhance sprouting of buds but increased the rate of multiplication when added in the medium. Maximum number of shoots were obtained when MS medium was supplemented with BAP (0.5 mg/L), GA3 (1 mg/L), IBA (0.1 mg/L) and PG (100 mg/L). For rooting, in vitro regenerated shoots were placed in IBA (30 mg/L) for 3 hr and transferred to solidified auxin free medium. Rooting was recorded in about 80% of shoots. Inclusion of PG in rooting medium was not beneficial but shoot cultures grown in its presence gave higher rooting percentage. Rooted plantlets showed about 70% survival rate in potting mixture of sand:soil:perlite (1:1:1).en_US
dc.description.affiliationDepartment of Biotechnology, Dr Y S Parmar University of Horticulture & Forestry, Nauni, Solan 173 230, India.en_US
dc.identifier.citationSharma M, Modgil M, Sharma DR. Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol. Indian Journal of Experimental Biology. 2000 Dec; 38(12): 1236-40en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/60385
dc.language.isoengen_US
dc.source.urihttps://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijeb/ijeb0.aspen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFruit --drug effectsen_US
dc.subject.meshIndiaen_US
dc.subject.meshPhloroglucinol --pharmacologyen_US
dc.subject.meshPlant Growth Regulators --pharmacologyen_US
dc.subject.meshPlant Roots --drug effectsen_US
dc.titleSuccessful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol.en_US
dc.typeJournal Articleen_US
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