Biofilm Production And Antifungal Resistance Among Candida Species In Patients With Vulvovaginitis: A Cross Sectional Study
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Date
2024-01
Journal Title
Journal ISSN
Volume Title
Publisher
Association of Health Professionals and Health Educators
Abstract
Background:Vulvovaginal candidiasis (VVC), one of the commonest fungal infections in women of reproductive age group, can become recurrent if not managed appropriately. Recently, production of biofilm, which reduces the susceptibility to anti-fungal agents, has also been implicated in its pathogenesis. Hence, thestudy was planned to determine the prevalence of VVC in cases of vulvovaginitis, identify the pre-dominant candida species,and find out their antifungal susceptibility pattern and to detect biofilm production in the candida isolates. Material And Methods:In this cross-sectional study, all isolates were identified and confirmed by various basic microbiological methods followed by the VITEK-2 system.Biofilm formation was detected by Congo red agar method and Tube method. Descriptive statistical methods were used to evaluate the data collected. Result:Out of total 25 patients, 7 were positive with VVC out of which 2 cases were caused by C.albicans and 5 by Candida Non Albicans (3 by C.glabrata and 2 by C.tropicalis). As compared to NAC, C. albicans is still partially sensitive to fluconazole. Fortunately, both C. albicans and C. tropicalis are still 100% sensitive to voriconazole although resistance has developed partially in C. glabrata. Biofilm production was observed in 3 cases. Conclusion:Majority of cases of VVC were due to NAC predominantly C. glabrata which has grave therapeutic implications as they show decreased susceptibility to all antifungals. Voriconazole is still effective but needs to be used rationally to avoid developing resistance to it also. The evidence of biofilm production could be an important contributor towards increased incidence ofantifungal drug resistance.
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Keywords
Antifungal Susceptibility Pattern, Biofilm, Candida Species, Vulvovaginal Candidiasis
Citation
Garg A, Kaur N.. Biofilm Production And Antifungal Resistance Among Candida Species In Patients With Vulvovaginitis: A Cross Sectional Study . Tropical Gastroenterology. 2024 Jan; 15(1): 19-25